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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polynucleobacter sp. Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP391842PYU
CSB-EP391842PYU
CSB-BP391842PYU
CSB-MP391842PYU
CSB-EP391842PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP391843PYU
CSB-EP391843PYU
CSB-BP391843PYU
CSB-MP391843PYU
CSB-EP391843PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Recombination protein RecR (recR) CSB-YP391844PYU
CSB-EP391844PYU
CSB-BP391844PYU
CSB-MP391844PYU
CSB-EP391844PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Putative arginyl-tRNA--protein transferase (ate) CSB-YP391845PYU
CSB-EP391845PYU
CSB-BP391845PYU
CSB-MP391845PYU
CSB-EP391845PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP391846PYU
CSB-EP391846PYU
CSB-BP391846PYU
CSB-MP391846PYU
CSB-EP391846PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Dihydrodipicolinate synthase (dapA) CSB-YP391847PYU
CSB-EP391847PYU
CSB-BP391847PYU
CSB-MP391847PYU
CSB-EP391847PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP391848PYU
CSB-EP391848PYU
CSB-BP391848PYU
CSB-MP391848PYU
CSB-EP391848PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP391849PYU
CSB-EP391849PYU
CSB-BP391849PYU
CSB-MP391849PYU
CSB-EP391849PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Enolase (eno) CSB-YP391850PYU
CSB-EP391850PYU
CSB-BP391850PYU
CSB-MP391850PYU
CSB-EP391850PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Probable intracellular septation protein A (Pnuc_0973), partial CSB-YP391851PYU1
CSB-EP391851PYU1
CSB-BP391851PYU1
CSB-MP391851PYU1
CSB-EP391851PYU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Phosphoglucosamine mutase (glmM) CSB-YP391852PYU
CSB-EP391852PYU
CSB-BP391852PYU
CSB-MP391852PYU
CSB-EP391852PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Carbamoyl-phosphate synthase small chain (carA) CSB-YP391853PYU
CSB-EP391853PYU
CSB-BP391853PYU
CSB-MP391853PYU
CSB-EP391853PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP391854PYU
CSB-EP391854PYU
CSB-BP391854PYU
CSB-MP391854PYU
CSB-EP391854PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Ketol-acid reductoisomerase (ilvC) CSB-YP391855PYU
CSB-EP391855PYU
CSB-BP391855PYU
CSB-MP391855PYU
CSB-EP391855PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Urease subunit alpha (ureC), partial CSB-YP391856PYU
CSB-EP391856PYU
CSB-BP391856PYU
CSB-MP391856PYU
CSB-EP391856PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Urease accessory protein UreD (ureD) CSB-YP391857PYU
CSB-EP391857PYU
CSB-BP391857PYU
CSB-MP391857PYU
CSB-EP391857PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Ribosome maturation factor RimP (rimP) CSB-YP391858PYU
CSB-EP391858PYU
CSB-BP391858PYU
CSB-MP391858PYU
CSB-EP391858PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Adenylosuccinate synthetase (purA) CSB-YP391859PYU
CSB-EP391859PYU
CSB-BP391859PYU
CSB-MP391859PYU
CSB-EP391859PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP391860PYU
CSB-EP391860PYU
CSB-BP391860PYU
CSB-MP391860PYU
CSB-EP391860PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP391861PYU
CSB-EP391861PYU
CSB-BP391861PYU
CSB-MP391861PYU
CSB-EP391861PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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