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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S15 (rpsO) CSB-YP392042GEH
CSB-EP392042GEH
CSB-BP392042GEH
CSB-MP392042GEH
CSB-EP392042GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans DNA mismatch repair protein MutS (mutS), partial CSB-YP392043GEH
CSB-EP392043GEH
CSB-BP392043GEH
CSB-MP392043GEH
CSB-EP392043GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans LexA repressor (lexA) CSB-YP392044GEH
CSB-EP392044GEH
CSB-BP392044GEH
CSB-MP392044GEH
CSB-EP392044GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Large-conductance mechanosensitive channel (mscL), partial CSB-YP392045GEH1
CSB-EP392045GEH1
CSB-BP392045GEH1
CSB-MP392045GEH1
CSB-EP392045GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0271 protein GTNG_1353 (GTNG_1353) CSB-YP392046GEH
CSB-EP392046GEH
CSB-BP392046GEH
CSB-MP392046GEH
CSB-EP392046GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Small, acid-soluble spore protein N (sspN) CSB-YP392047GEH
CSB-EP392047GEH
CSB-BP392047GEH
CSB-MP392047GEH
CSB-EP392047GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0346 protein GTNG_1419 (GTNG_1419) CSB-YP392048GEH
CSB-EP392048GEH
CSB-BP392048GEH
CSB-MP392048GEH
CSB-EP392048GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Protein ArsC (arsC) CSB-YP392049GEH
CSB-EP392049GEH
CSB-BP392049GEH
CSB-MP392049GEH
CSB-EP392049GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP392050GEH
CSB-EP392050GEH
CSB-BP392050GEH
CSB-MP392050GEH
CSB-EP392050GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Mannonate dehydratase (uxuA) CSB-YP392051GEH
CSB-EP392051GEH
CSB-BP392051GEH
CSB-MP392051GEH
CSB-EP392051GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ribulokinase (araB), partial CSB-YP392052GEH
CSB-EP392052GEH
CSB-BP392052GEH
CSB-MP392052GEH
CSB-EP392052GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 5-deoxy-glucuronate isomerase (iolB) CSB-YP392053GEH
CSB-EP392053GEH
CSB-BP392053GEH
CSB-MP392053GEH
CSB-EP392053GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Glutamate 5-kinase (proB) CSB-YP392054GEH
CSB-EP392054GEH
CSB-BP392054GEH
CSB-MP392054GEH
CSB-EP392054GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Holliday junction resolvase recU (recU) CSB-YP392055GEH
CSB-EP392055GEH
CSB-BP392055GEH
CSB-MP392055GEH
CSB-EP392055GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP392056GEH
CSB-EP392056GEH
CSB-BP392056GEH
CSB-MP392056GEH
CSB-EP392056GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Methylglyoxal synthase (mgsA) CSB-YP392057GEH
CSB-EP392057GEH
CSB-BP392057GEH
CSB-MP392057GEH
CSB-EP392057GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP392058GEH
CSB-EP392058GEH
CSB-BP392058GEH
CSB-MP392058GEH
CSB-EP392058GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 3-dehydroquinate synthase (aroB) CSB-YP392059GEH
CSB-EP392059GEH
CSB-BP392059GEH
CSB-MP392059GEH
CSB-EP392059GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Cobalamin synthase (cobS), partial CSB-YP392060GEH1
CSB-EP392060GEH1
CSB-BP392060GEH1
CSB-MP392060GEH1
CSB-EP392060GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP392061GEH
CSB-EP392061GEH
CSB-BP392061GEH
CSB-MP392061GEH
CSB-EP392061GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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