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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aethionema cordifolium Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP391442AUJ
CSB-EP391442AUJ
CSB-BP391442AUJ
CSB-MP391442AUJ
CSB-EP391442AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP391443AUJ1
CSB-EP391443AUJ1
CSB-BP391443AUJ1
CSB-MP391443AUJ1
CSB-EP391443AUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP391444AUJ
CSB-EP391444AUJ
CSB-BP391444AUJ
CSB-MP391444AUJ
CSB-EP391444AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium Photosystem II reaction center protein J (psbJ), partial CSB-YP391445AUJ
CSB-EP391445AUJ
CSB-BP391445AUJ
CSB-MP391445AUJ
CSB-EP391445AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP391446AUJ
CSB-EP391446AUJ
CSB-BP391446AUJ
CSB-MP391446AUJ
CSB-EP391446AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP391447AUJ
CSB-EP391447AUJ
CSB-BP391447AUJ
CSB-MP391447AUJ
CSB-EP391447AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP391448AUJ
CSB-EP391448AUJ
CSB-BP391448AUJ
CSB-MP391448AUJ
CSB-EP391448AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP391449AUJ
CSB-EP391449AUJ
CSB-BP391449AUJ
CSB-MP391449AUJ
CSB-EP391449AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP391450AUJ
CSB-EP391450AUJ
CSB-BP391450AUJ
CSB-MP391450AUJ
CSB-EP391450AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP391451AUJ
CSB-EP391451AUJ
CSB-BP391451AUJ
CSB-MP391451AUJ
CSB-EP391451AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP391452AUJ
CSB-EP391452AUJ
CSB-BP391452AUJ
CSB-MP391452AUJ
CSB-EP391452AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium NAD (P)H-quinone oxidoreductase chain 4, chloroplastic, partial CSB-YP391453AUJ
CSB-EP391453AUJ
CSB-BP391453AUJ
CSB-MP391453AUJ
CSB-EP391453AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium Photosystem I iron-sulfur center (psaC) CSB-YP391454AUJ
CSB-EP391454AUJ
CSB-BP391454AUJ
CSB-MP391454AUJ
CSB-EP391454AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP391455AUJ
CSB-EP391455AUJ
CSB-BP391455AUJ
CSB-MP391455AUJ
CSB-EP391455AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema cordifolium Putative membrane protein ycf1 (ycf1-A), partial CSB-YP391456AUJ
CSB-EP391456AUJ
CSB-BP391456AUJ
CSB-MP391456AUJ
CSB-EP391456AUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema grandiflora Photosystem Q (B) protein, partial CSB-YP391457AUK1
CSB-EP391457AUK1
CSB-BP391457AUK1
CSB-MP391457AUK1
CSB-EP391457AUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema grandiflora ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP391458AUK1
CSB-EP391458AUK1
CSB-BP391458AUK1
CSB-MP391458AUK1
CSB-EP391458AUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema grandiflora 30S ribosomal protein S2, chloroplastic (rps2) CSB-YP391459AUK
CSB-EP391459AUK
CSB-BP391459AUK
CSB-MP391459AUK
CSB-EP391459AUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema grandiflora DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP391460AUK
CSB-EP391460AUK
CSB-BP391460AUK
CSB-MP391460AUK
CSB-EP391460AUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aethionema grandiflora Photosystem II reaction center protein M (psbM), partial CSB-YP391461AUK
CSB-EP391461AUK
CSB-BP391461AUK
CSB-MP391461AUK
CSB-EP391461AUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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