Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacillus thermodenitrificans Glycogen synthase (glgA) CSB-YP392082GEH
CSB-EP392082GEH
CSB-BP392082GEH
CSB-MP392082GEH
CSB-EP392082GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Metallothiol transferase fosB (fosB) CSB-YP392083GEH
CSB-EP392083GEH
CSB-BP392083GEH
CSB-MP392083GEH
CSB-EP392083GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ferredoxin--NADP reductase (GTNG_2905) CSB-YP392084GEH
CSB-EP392084GEH
CSB-BP392084GEH
CSB-MP392084GEH
CSB-EP392084GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans SsrA-binding protein (smpB) CSB-YP392085GEH
CSB-EP392085GEH
CSB-BP392085GEH
CSB-MP392085GEH
CSB-EP392085GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP392086GEH
CSB-EP392086GEH
CSB-BP392086GEH
CSB-MP392086GEH
CSB-EP392086GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans HPr kinase/phosphorylase (hprK) CSB-YP392087GEH
CSB-EP392087GEH
CSB-BP392087GEH
CSB-MP392087GEH
CSB-EP392087GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Protein translocase subunit SecA 2 (secA2), partial CSB-YP392088GEH
CSB-EP392088GEH
CSB-BP392088GEH
CSB-MP392088GEH
CSB-EP392088GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Acetaldehyde dehydrogenase (nbaJ) CSB-YP392089GEH
CSB-EP392089GEH
CSB-BP392089GEH
CSB-MP392089GEH
CSB-EP392089GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP392090GEH1
CSB-EP392090GEH1
CSB-BP392090GEH1
CSB-MP392090GEH1
CSB-EP392090GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP392091GEH1
CSB-EP392091GEH1
CSB-BP392091GEH1
CSB-MP392091GEH1
CSB-EP392091GEH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Arginine--tRNA ligase (argS), partial CSB-YP392092GEH
CSB-EP392092GEH
CSB-BP392092GEH
CSB-MP392092GEH
CSB-EP392092GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Nucleoid occlusion protein (noc) CSB-YP392093GEH
CSB-EP392093GEH
CSB-BP392093GEH
CSB-MP392093GEH
CSB-EP392093GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP392094GEH
CSB-EP392094GEH
CSB-BP392094GEH
CSB-MP392094GEH
CSB-EP392094GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Ribonuclease P protein component (rnpA) CSB-YP392095GEH
CSB-EP392095GEH
CSB-BP392095GEH
CSB-MP392095GEH
CSB-EP392095GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Na (+)/H (+) antiporter nhaA, partial CSB-YP392096FDX
CSB-EP392096FDX
CSB-BP392096FDX
CSB-MP392096FDX
CSB-EP392096FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP392097FDX
CSB-EP392097FDX
CSB-BP392097FDX
CSB-MP392097FDX
CSB-EP392097FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP392098FDX
CSB-EP392098FDX
CSB-BP392098FDX
CSB-MP392098FDX
CSB-EP392098FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Peptide chain release factor 3 (prfC), partial CSB-YP392099FDX
CSB-EP392099FDX
CSB-BP392099FDX
CSB-MP392099FDX
CSB-EP392099FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis Glycerol kinase (glpK), partial CSB-YP392100FDX
CSB-EP392100FDX
CSB-BP392100FDX
CSB-MP392100FDX
CSB-EP392100FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. tularensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP392101FDX
CSB-EP392101FDX
CSB-BP392101FDX
CSB-MP392101FDX
CSB-EP392101FDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>