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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfotomaculum reducens 2-isopropylmalate synthase (leuA), partial CSB-YP392162DHX
CSB-EP392162DHX
CSB-BP392162DHX
CSB-MP392162DHX
CSB-EP392162DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Hydroxyethylthiazole kinase (thiM) CSB-YP392163DHX
CSB-EP392163DHX
CSB-BP392163DHX
CSB-MP392163DHX
CSB-EP392163DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Protein MraZ (mraZ) CSB-YP392164DHX
CSB-EP392164DHX
CSB-BP392164DHX
CSB-MP392164DHX
CSB-EP392164DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP392165DHX
CSB-EP392165DHX
CSB-BP392165DHX
CSB-MP392165DHX
CSB-EP392165DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP392166DHX
CSB-EP392166DHX
CSB-BP392166DHX
CSB-MP392166DHX
CSB-EP392166DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Cell division protein SepF 1 (sepF1) CSB-YP392167DHX
CSB-EP392167DHX
CSB-BP392167DHX
CSB-MP392167DHX
CSB-EP392167DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP392168DHX
CSB-EP392168DHX
CSB-BP392168DHX
CSB-MP392168DHX
CSB-EP392168DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Histidine--tRNA ligase (hisS) CSB-YP392169DHX
CSB-EP392169DHX
CSB-BP392169DHX
CSB-MP392169DHX
CSB-EP392169DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UPF0473 protein Dred_0776 (Dred_0776) CSB-YP392170DHX
CSB-EP392170DHX
CSB-BP392170DHX
CSB-MP392170DHX
CSB-EP392170DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP392171DHX
CSB-EP392171DHX
CSB-BP392171DHX
CSB-MP392171DHX
CSB-EP392171DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Elongation factor P (efp) CSB-YP392172DHX
CSB-EP392172DHX
CSB-BP392172DHX
CSB-MP392172DHX
CSB-EP392172DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP392173DHX
CSB-EP392173DHX
CSB-BP392173DHX
CSB-MP392173DHX
CSB-EP392173DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP392174DHX
CSB-EP392174DHX
CSB-BP392174DHX
CSB-MP392174DHX
CSB-EP392174DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens GTPase Der (der) CSB-YP392175DHX
CSB-EP392175DHX
CSB-BP392175DHX
CSB-MP392175DHX
CSB-EP392175DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 33 kDa chaperonin (hslO) CSB-YP392176DHX
CSB-EP392176DHX
CSB-BP392176DHX
CSB-MP392176DHX
CSB-EP392176DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Glycogen synthase (glgA) CSB-YP392177DHX
CSB-EP392177DHX
CSB-BP392177DHX
CSB-MP392177DHX
CSB-EP392177DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Threonine--tRNA ligase (thrS), partial CSB-YP392178DHX
CSB-EP392178DHX
CSB-BP392178DHX
CSB-MP392178DHX
CSB-EP392178DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP392179DHX
CSB-EP392179DHX
CSB-BP392179DHX
CSB-MP392179DHX
CSB-EP392179DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP392180DHX
CSB-EP392180DHX
CSB-BP392180DHX
CSB-MP392180DHX
CSB-EP392180DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP392181DHX
CSB-EP392181DHX
CSB-BP392181DHX
CSB-MP392181DHX
CSB-EP392181DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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