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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacillus thermodenitrificans Putative septation protein spoVG (spoVG) CSB-YP392002GEH
CSB-EP392002GEH
CSB-BP392002GEH
CSB-MP392002GEH
CSB-EP392002GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Peptidyl-tRNA hydrolase (pth) CSB-YP392003GEH
CSB-EP392003GEH
CSB-BP392003GEH
CSB-MP392003GEH
CSB-EP392003GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP392004GEH
CSB-EP392004GEH
CSB-BP392004GEH
CSB-MP392004GEH
CSB-EP392004GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Putative ribosomal protein L7Ae-like (GTNG_0100) CSB-YP392005GEH
CSB-EP392005GEH
CSB-BP392005GEH
CSB-MP392005GEH
CSB-EP392005GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S10 (rpsJ) CSB-YP392006GEH
CSB-EP392006GEH
CSB-BP392006GEH
CSB-MP392006GEH
CSB-EP392006GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S19 (rpsS) CSB-YP392007GEH
CSB-EP392007GEH
CSB-BP392007GEH
CSB-MP392007GEH
CSB-EP392007GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L16 (rplP) CSB-YP392008GEH
CSB-EP392008GEH
CSB-BP392008GEH
CSB-MP392008GEH
CSB-EP392008GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 30S ribosomal protein S17 (rpsQ) CSB-YP392009GEH
CSB-EP392009GEH
CSB-BP392009GEH
CSB-MP392009GEH
CSB-EP392009GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L18 (rplR) CSB-YP392010GEH
CSB-EP392010GEH
CSB-BP392010GEH
CSB-MP392010GEH
CSB-EP392010GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 50S ribosomal protein L15 (rplO) CSB-YP392011GEH
CSB-EP392011GEH
CSB-BP392011GEH
CSB-MP392011GEH
CSB-EP392011GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Adenylate kinase (adk) CSB-YP392012GEH
CSB-EP392012GEH
CSB-BP392012GEH
CSB-MP392012GEH
CSB-EP392012GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Phosphoglucosamine mutase (glmM) CSB-YP392013GEH
CSB-EP392013GEH
CSB-BP392013GEH
CSB-MP392013GEH
CSB-EP392013GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP392014GEH
CSB-EP392014GEH
CSB-BP392014GEH
CSB-MP392014GEH
CSB-EP392014GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans 60 kDa chaperonin (groL), partial CSB-YP392015GEH
CSB-EP392015GEH
CSB-BP392015GEH
CSB-MP392015GEH
CSB-EP392015GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP392016GEH
CSB-EP392016GEH
CSB-BP392016GEH
CSB-MP392016GEH
CSB-EP392016GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP392017GEH
CSB-EP392017GEH
CSB-BP392017GEH
CSB-MP392017GEH
CSB-EP392017GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP392018GEH
CSB-EP392018GEH
CSB-BP392018GEH
CSB-MP392018GEH
CSB-EP392018GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Hut operon positive regulatory protein (hutP) CSB-YP392019GEH
CSB-EP392019GEH
CSB-BP392019GEH
CSB-MP392019GEH
CSB-EP392019GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Lactate utilization protein A (lutA) CSB-YP392020GEH
CSB-EP392020GEH
CSB-BP392020GEH
CSB-MP392020GEH
CSB-EP392020GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans UPF0753 protein GTNG_0448 (GTNG_0448), partial CSB-YP392021GEH
CSB-EP392021GEH
CSB-BP392021GEH
CSB-MP392021GEH
CSB-EP392021GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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