Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Burkholderia vietnamiensis Protein CyaY (cyaY) CSB-YP392222BPV
CSB-EP392222BPV
CSB-BP392222BPV
CSB-MP392222BPV
CSB-EP392222BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP392223BPV
CSB-EP392223BPV
CSB-BP392223BPV
CSB-MP392223BPV
CSB-EP392223BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP392224BPV
CSB-EP392224BPV
CSB-BP392224BPV
CSB-MP392224BPV
CSB-EP392224BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Anthranilate phosphoribosyltransferase (trpD) CSB-YP392225BPV
CSB-EP392225BPV
CSB-BP392225BPV
CSB-MP392225BPV
CSB-EP392225BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP392226BPV
CSB-EP392226BPV
CSB-BP392226BPV
CSB-MP392226BPV
CSB-EP392226BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP392227BPV
CSB-EP392227BPV
CSB-BP392227BPV
CSB-MP392227BPV
CSB-EP392227BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP392228BPV
CSB-EP392228BPV
CSB-BP392228BPV
CSB-MP392228BPV
CSB-EP392228BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0289 protein Bcep1808_0549 (Bcep1808_0549) CSB-YP392229BPV
CSB-EP392229BPV
CSB-BP392229BPV
CSB-MP392229BPV
CSB-EP392229BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Dihydrodipicolinate reductase (dapB) CSB-YP392230BPV
CSB-EP392230BPV
CSB-BP392230BPV
CSB-MP392230BPV
CSB-EP392230BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L13 (rplM) CSB-YP392231BPV
CSB-EP392231BPV
CSB-BP392231BPV
CSB-MP392231BPV
CSB-EP392231BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP392232BPV
CSB-EP392232BPV
CSB-BP392232BPV
CSB-MP392232BPV
CSB-EP392232BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP392233BPV
CSB-EP392233BPV
CSB-BP392233BPV
CSB-MP392233BPV
CSB-EP392233BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Protein grpE (grpE) CSB-YP392234BPV
CSB-EP392234BPV
CSB-BP392234BPV
CSB-MP392234BPV
CSB-EP392234BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis D- (-)-3-hydroxybutyrate oligomer hydrolase, partial CSB-YP392235BPV
CSB-EP392235BPV
CSB-BP392235BPV
CSB-MP392235BPV
CSB-EP392235BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Urease accessory protein UreF (ureF) CSB-YP392236BPV
CSB-EP392236BPV
CSB-BP392236BPV
CSB-MP392236BPV
CSB-EP392236BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Urease accessory protein UreD (ureD) CSB-YP392237BPV
CSB-EP392237BPV
CSB-BP392237BPV
CSB-MP392237BPV
CSB-EP392237BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP392238BPV
CSB-EP392238BPV
CSB-BP392238BPV
CSB-MP392238BPV
CSB-EP392238BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Probable septum site-determining protein MinC (minC) CSB-YP392239BPV
CSB-EP392239BPV
CSB-BP392239BPV
CSB-MP392239BPV
CSB-EP392239BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Ribonuclease PH (rph) CSB-YP392240BPV
CSB-EP392240BPV
CSB-BP392240BPV
CSB-MP392240BPV
CSB-EP392240BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP392241BPV
CSB-EP392241BPV
CSB-BP392241BPV
CSB-MP392241BPV
CSB-EP392241BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>