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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Polynucleobacter sp. Ribose-5-phosphate isomerase A (rpiA) CSB-YP392762PYU
CSB-EP392762PYU
CSB-BP392762PYU
CSB-MP392762PYU
CSB-EP392762PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP392763PYU
CSB-EP392763PYU
CSB-BP392763PYU
CSB-MP392763PYU
CSB-EP392763PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L31 type B (rpmE2) CSB-YP392764PYU
CSB-EP392764PYU
CSB-BP392764PYU
CSB-MP392764PYU
CSB-EP392764PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. SsrA-binding protein (smpB) CSB-YP392765PYU
CSB-EP392765PYU
CSB-BP392765PYU
CSB-MP392765PYU
CSB-EP392765PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP392766PYU
CSB-EP392766PYU
CSB-BP392766PYU
CSB-MP392766PYU
CSB-EP392766PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S2 (rpsB) CSB-YP392767PYU
CSB-EP392767PYU
CSB-BP392767PYU
CSB-MP392767PYU
CSB-EP392767PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP392768PYU
CSB-EP392768PYU
CSB-BP392768PYU
CSB-MP392768PYU
CSB-EP392768PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Potassium-transporting ATPase C chain (kdpC), partial CSB-YP392769PYU1
CSB-EP392769PYU1
CSB-BP392769PYU1
CSB-MP392769PYU1
CSB-EP392769PYU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Alanine--tRNA ligase (alaS), partial CSB-YP392770PYU
CSB-EP392770PYU
CSB-BP392770PYU
CSB-MP392770PYU
CSB-EP392770PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L28 (rpmB) CSB-YP392771PYU
CSB-EP392771PYU
CSB-BP392771PYU
CSB-MP392771PYU
CSB-EP392771PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 30S ribosomal protein S20 (rpsT) CSB-YP392772PYU
CSB-EP392772PYU
CSB-BP392772PYU
CSB-MP392772PYU
CSB-EP392772PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Chaperone protein DnaK (dnaK), partial CSB-YP392773PYU
CSB-EP392773PYU
CSB-BP392773PYU
CSB-MP392773PYU
CSB-EP392773PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 60 kDa chaperonin (groL), partial CSB-YP392774PYU
CSB-EP392774PYU
CSB-BP392774PYU
CSB-MP392774PYU
CSB-EP392774PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L13 (rplM) CSB-YP392775PYU
CSB-EP392775PYU
CSB-BP392775PYU
CSB-MP392775PYU
CSB-EP392775PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. 50S ribosomal protein L25 (rplY) CSB-YP392776PYU
CSB-EP392776PYU
CSB-BP392776PYU
CSB-MP392776PYU
CSB-EP392776PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP392777PYU
CSB-EP392777PYU
CSB-BP392777PYU
CSB-MP392777PYU
CSB-EP392777PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Cobyric acid synthase (cobQ) CSB-YP392778MOK
CSB-EP392778MOK
CSB-BP392778MOK
CSB-MP392778MOK
CSB-EP392778MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Chaperone protein DnaK (dnaK), partial CSB-YP392779MOK
CSB-EP392779MOK
CSB-BP392779MOK
CSB-MP392779MOK
CSB-EP392779MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum F420-dependent glucose-6-phosphate dehydrogenase (fgd) CSB-YP392780MOK
CSB-EP392780MOK
CSB-BP392780MOK
CSB-MP392780MOK
CSB-EP392780MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP392781MOK
CSB-EP392781MOK
CSB-BP392781MOK
CSB-MP392781MOK
CSB-EP392781MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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