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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfotomaculum reducens Sugar fermentation stimulation protein homolog (sfsA) CSB-YP392202DHX
CSB-EP392202DHX
CSB-BP392202DHX
CSB-MP392202DHX
CSB-EP392202DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Maf-like protein Dred_2550 (Dred_2550) CSB-YP392203DHX
CSB-EP392203DHX
CSB-BP392203DHX
CSB-MP392203DHX
CSB-EP392203DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Ribonuclease PH (rph) CSB-YP392204DHX
CSB-EP392204DHX
CSB-BP392204DHX
CSB-MP392204DHX
CSB-EP392204DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Cobalt transport protein CbiM (cbiM), partial CSB-YP392205DHX1
CSB-EP392205DHX1
CSB-BP392205DHX1
CSB-MP392205DHX1
CSB-EP392205DHX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Alanine racemase (alr) CSB-YP392206DHX
CSB-EP392206DHX
CSB-BP392206DHX
CSB-MP392206DHX
CSB-EP392206DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens SsrA-binding protein (smpB) CSB-YP392207DHX
CSB-EP392207DHX
CSB-BP392207DHX
CSB-MP392207DHX
CSB-EP392207DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens S-adenosylmethionine synthase (metK) CSB-YP392208DHX
CSB-EP392208DHX
CSB-BP392208DHX
CSB-MP392208DHX
CSB-EP392208DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Probable transaldolase (tal) CSB-YP392209DHX
CSB-EP392209DHX
CSB-BP392209DHX
CSB-MP392209DHX
CSB-EP392209DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 30S ribosomal protein S6 (rpsF) CSB-YP392210DHX
CSB-EP392210DHX
CSB-BP392210DHX
CSB-MP392210DHX
CSB-EP392210DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Ribonuclease P protein component (rnpA) CSB-YP392211DHX
CSB-EP392211DHX
CSB-BP392211DHX
CSB-MP392211DHX
CSB-EP392211DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP392212BPV
CSB-EP392212BPV
CSB-BP392212BPV
CSB-MP392212BPV
CSB-EP392212BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis UPF0102 protein Bcep1808_0248 (Bcep1808_0248) CSB-YP392213BPV
CSB-EP392213BPV
CSB-BP392213BPV
CSB-MP392213BPV
CSB-EP392213BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L7/L12 (rplL) CSB-YP392214BPV
CSB-EP392214BPV
CSB-BP392214BPV
CSB-MP392214BPV
CSB-EP392214BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP392215BPV
CSB-EP392215BPV
CSB-BP392215BPV
CSB-MP392215BPV
CSB-EP392215BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 30S ribosomal protein S7 (rpsG) CSB-YP392216BPV
CSB-EP392216BPV
CSB-BP392216BPV
CSB-MP392216BPV
CSB-EP392216BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L22 (rplV) CSB-YP392217BPV
CSB-EP392217BPV
CSB-BP392217BPV
CSB-MP392217BPV
CSB-EP392217BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L14 (rplN) CSB-YP392218BPV
CSB-EP392218BPV
CSB-BP392218BPV
CSB-MP392218BPV
CSB-EP392218BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L18 (rplR) CSB-YP392219BPV
CSB-EP392219BPV
CSB-BP392219BPV
CSB-MP392219BPV
CSB-EP392219BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Translation initiation factor IF-1 1 (infA1) CSB-YP392220BPV
CSB-EP392220BPV
CSB-BP392220BPV
CSB-MP392220BPV
CSB-EP392220BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP392221BPV
CSB-EP392221BPV
CSB-BP392221BPV
CSB-MP392221BPV
CSB-EP392221BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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