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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium gilvum Urease accessory protein UreG (ureG) CSB-YP392802MOK
CSB-EP392802MOK
CSB-BP392802MOK
CSB-MP392802MOK
CSB-EP392802MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP392803MOK
CSB-EP392803MOK
CSB-BP392803MOK
CSB-MP392803MOK
CSB-EP392803MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 4-hydroxy-2-oxovalerate aldolase (Mflv_1522) CSB-YP392804MOK
CSB-EP392804MOK
CSB-BP392804MOK
CSB-MP392804MOK
CSB-EP392804MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0286 protein Mflv_2337 (Mflv_2337) CSB-YP392805MOK
CSB-EP392805MOK
CSB-BP392805MOK
CSB-MP392805MOK
CSB-EP392805MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP synthase epsilon chain (atpC) CSB-YP392806MOK
CSB-EP392806MOK
CSB-BP392806MOK
CSB-MP392806MOK
CSB-EP392806MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Arginine--tRNA ligase (argS), partial CSB-YP392807MOK
CSB-EP392807MOK
CSB-BP392807MOK
CSB-MP392807MOK
CSB-EP392807MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Sec-independent protein translocase protein TatB (tatB), partial CSB-YP392808MOK
CSB-EP392808MOK
CSB-BP392808MOK
CSB-MP392808MOK
CSB-EP392808MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP392809MOK
CSB-EP392809MOK
CSB-BP392809MOK
CSB-MP392809MOK
CSB-EP392809MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Biotin synthase (bioB) CSB-YP392810MOK
CSB-EP392810MOK
CSB-BP392810MOK
CSB-MP392810MOK
CSB-EP392810MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Indole-3-glycerol phosphate synthase (trpC) CSB-YP392811MOK
CSB-EP392811MOK
CSB-BP392811MOK
CSB-MP392811MOK
CSB-EP392811MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L35 (rpmI) CSB-YP392812MOK
CSB-EP392812MOK
CSB-BP392812MOK
CSB-MP392812MOK
CSB-EP392812MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Malate synthase G (glcB), partial CSB-YP392813MOK
CSB-EP392813MOK
CSB-BP392813MOK
CSB-MP392813MOK
CSB-EP392813MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0060 membrane protein Mflv_3127 (Mflv_3127), partial CSB-YP392814MOK1
CSB-EP392814MOK1
CSB-BP392814MOK1
CSB-MP392814MOK1
CSB-EP392814MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Pup--protein ligase (pafA) CSB-YP392815MOK
CSB-EP392815MOK
CSB-BP392815MOK
CSB-MP392815MOK
CSB-EP392815MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP392816MOK
CSB-EP392816MOK
CSB-BP392816MOK
CSB-MP392816MOK
CSB-EP392816MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Protein MraZ (mraZ) CSB-YP392817MOK
CSB-EP392817MOK
CSB-BP392817MOK
CSB-MP392817MOK
CSB-EP392817MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Octanoyltransferase (lipB) CSB-YP392818MOK
CSB-EP392818MOK
CSB-BP392818MOK
CSB-MP392818MOK
CSB-EP392818MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum N utilization substance protein B homolog (nusB) CSB-YP392819MOK
CSB-EP392819MOK
CSB-BP392819MOK
CSB-MP392819MOK
CSB-EP392819MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum S-adenosylmethionine synthase (metK) CSB-YP392820MOK
CSB-EP392820MOK
CSB-BP392820MOK
CSB-MP392820MOK
CSB-EP392820MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP392821MOK
CSB-EP392821MOK
CSB-BP392821MOK
CSB-MP392821MOK
CSB-EP392821MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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