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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus suis UPF0397 protein SSU98_0390 (SSU98_0390), partial CSB-YP394303SUT1
CSB-EP394303SUT1
CSB-BP394303SUT1
CSB-MP394303SUT1
CSB-EP394303SUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribonuclease Y (rny), partial CSB-YP394304SUT
CSB-EP394304SUT
CSB-BP394304SUT
CSB-MP394304SUT
CSB-EP394304SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP394305SUT
CSB-EP394305SUT
CSB-BP394305SUT
CSB-MP394305SUT
CSB-EP394305SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Initiation-control protein yabA (SSU98_0663) CSB-YP394306SUT
CSB-EP394306SUT
CSB-BP394306SUT
CSB-MP394306SUT
CSB-EP394306SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP394307SUT
CSB-EP394307SUT
CSB-BP394307SUT
CSB-MP394307SUT
CSB-EP394307SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Aspartate carbamoyltransferase (pyrB) CSB-YP394308SUT
CSB-EP394308SUT
CSB-BP394308SUT
CSB-MP394308SUT
CSB-EP394308SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP394309SUT
CSB-EP394309SUT
CSB-BP394309SUT
CSB-MP394309SUT
CSB-EP394309SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Galactose-6-phosphate isomerase subunit lacB (lacB) CSB-YP394310SUT
CSB-EP394310SUT
CSB-BP394310SUT
CSB-MP394310SUT
CSB-EP394310SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S20 (rpsT) CSB-YP394311SUT
CSB-EP394311SUT
CSB-BP394311SUT
CSB-MP394311SUT
CSB-EP394311SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0342 protein SSU98_1275 (SSU98_1275) CSB-YP394312SUT
CSB-EP394312SUT
CSB-BP394312SUT
CSB-MP394312SUT
CSB-EP394312SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0346 protein SSU98_1338 (SSU98_1338) CSB-YP394313SUT
CSB-EP394313SUT
CSB-BP394313SUT
CSB-MP394313SUT
CSB-EP394313SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis tRNA modification GTPase MnmE (mnmE) CSB-YP394314SUT
CSB-EP394314SUT
CSB-BP394314SUT
CSB-MP394314SUT
CSB-EP394314SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis FMN-dependent NADH-azoreductase (azoR) CSB-YP394315SUT
CSB-EP394315SUT
CSB-BP394315SUT
CSB-MP394315SUT
CSB-EP394315SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP394316SUT
CSB-EP394316SUT
CSB-BP394316SUT
CSB-MP394316SUT
CSB-EP394316SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis GTPase Der (der) CSB-YP394317SUT
CSB-EP394317SUT
CSB-BP394317SUT
CSB-MP394317SUT
CSB-EP394317SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP394318SUT
CSB-EP394318SUT
CSB-BP394318SUT
CSB-MP394318SUT
CSB-EP394318SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribosome maturation factor RimP (rimP) CSB-YP394319SUT
CSB-EP394319SUT
CSB-BP394319SUT
CSB-MP394319SUT
CSB-EP394319SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis DNA mismatch repair protein MutS (mutS), partial CSB-YP394320SUT
CSB-EP394320SUT
CSB-BP394320SUT
CSB-MP394320SUT
CSB-EP394320SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Orotate phosphoribosyltransferase (pyrE) CSB-YP394321EIZ
CSB-EP394321EIZ
CSB-BP394321EIZ
CSB-MP394321EIZ
CSB-EP394321EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein-export protein secB (secB) CSB-YP394322EIZ
CSB-EP394322EIZ
CSB-BP394322EIZ
CSB-MP394322EIZ
CSB-EP394322EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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