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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfotomaculum reducens Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP393102DHX
CSB-EP393102DHX
CSB-BP393102DHX
CSB-MP393102DHX
CSB-EP393102DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Probable rRNA maturation factor (Dred_2482) CSB-YP393103DHX
CSB-EP393103DHX
CSB-BP393103DHX
CSB-MP393103DHX
CSB-EP393103DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Heat-inducible transcription repressor HrcA (hrcA) CSB-YP393104DHX
CSB-EP393104DHX
CSB-BP393104DHX
CSB-MP393104DHX
CSB-EP393104DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens GTPase obg (obg) CSB-YP393105DHX
CSB-EP393105DHX
CSB-BP393105DHX
CSB-MP393105DHX
CSB-EP393105DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Cell division topological specificity factor (minE) CSB-YP393106DHX
CSB-EP393106DHX
CSB-BP393106DHX
CSB-MP393106DHX
CSB-EP393106DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Redox-sensing transcriptional repressor rex (rex) CSB-YP393107DHX
CSB-EP393107DHX
CSB-BP393107DHX
CSB-MP393107DHX
CSB-EP393107DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Cobalamin synthase (cobS), partial CSB-YP393108DHX1
CSB-EP393108DHX1
CSB-BP393108DHX1
CSB-MP393108DHX1
CSB-EP393108DHX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens Nitrogenase iron protein (nifH) CSB-YP393109DHX
CSB-EP393109DHX
CSB-BP393109DHX
CSB-MP393109DHX
CSB-EP393109DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 60 kDa chaperonin (groL), partial CSB-YP393110DHX
CSB-EP393110DHX
CSB-BP393110DHX
CSB-MP393110DHX
CSB-EP393110DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UPF0042 nucleotide-binding protein Dred_3054 (Dred_3054) CSB-YP393111DHX
CSB-EP393111DHX
CSB-BP393111DHX
CSB-MP393111DHX
CSB-EP393111DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP393112DHX
CSB-EP393112DHX
CSB-BP393112DHX
CSB-MP393112DHX
CSB-EP393112DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens ATP synthase gamma chain (atpG) CSB-YP393113DHX
CSB-EP393113DHX
CSB-BP393113DHX
CSB-MP393113DHX
CSB-EP393113DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens UPF0059 membrane protein Dred_3165 (Dred_3165), partial CSB-YP393114DHX1
CSB-EP393114DHX1
CSB-BP393114DHX1
CSB-MP393114DHX1
CSB-EP393114DHX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens 50S ribosomal protein L9 (rplI) CSB-YP393115DHX
CSB-EP393115DHX
CSB-BP393115DHX
CSB-MP393115DHX
CSB-EP393115DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotomaculum reducens tRNA modification GTPase MnmE (mnmE) CSB-YP393116DHX
CSB-EP393116DHX
CSB-BP393116DHX
CSB-MP393116DHX
CSB-EP393116DHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Putative pterin-4-alpha-carbinolamine dehydratase (Bcep1808_0087) CSB-YP393117BPV
CSB-EP393117BPV
CSB-BP393117BPV
CSB-MP393117BPV
CSB-EP393117BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP393118BPV
CSB-EP393118BPV
CSB-BP393118BPV
CSB-MP393118BPV
CSB-EP393118BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L1 (rplA) CSB-YP393119BPV
CSB-EP393119BPV
CSB-BP393119BPV
CSB-MP393119BPV
CSB-EP393119BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L3 (rplC) CSB-YP393120BPV
CSB-EP393120BPV
CSB-BP393120BPV
CSB-MP393120BPV
CSB-EP393120BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia vietnamiensis 50S ribosomal protein L23 (rplW) CSB-YP393121BPV
CSB-EP393121BPV
CSB-BP393121BPV
CSB-MP393121BPV
CSB-EP393121BPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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