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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prosthecochloris vibrioformis 30S ribosomal protein S5 (rpsE) CSB-YP393502PZJ
CSB-EP393502PZJ
CSB-BP393502PZJ
CSB-MP393502PZJ
CSB-EP393502PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP393503PZJ
CSB-EP393503PZJ
CSB-BP393503PZJ
CSB-MP393503PZJ
CSB-EP393503PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Light-independent protochlorophyllide reductase subunit B (bchB), partial CSB-YP393504PZJ
CSB-EP393504PZJ
CSB-BP393504PZJ
CSB-MP393504PZJ
CSB-EP393504PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP393505PZJ
CSB-EP393505PZJ
CSB-BP393505PZJ
CSB-MP393505PZJ
CSB-EP393505PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP393506PZJ
CSB-EP393506PZJ
CSB-BP393506PZJ
CSB-MP393506PZJ
CSB-EP393506PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Dihydrodipicolinate reductase (dapB) CSB-YP393507PZJ
CSB-EP393507PZJ
CSB-BP393507PZJ
CSB-MP393507PZJ
CSB-EP393507PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis UPF0747 protein Cvib_0419 (Cvib_0419), partial CSB-YP393508PZJ
CSB-EP393508PZJ
CSB-BP393508PZJ
CSB-MP393508PZJ
CSB-EP393508PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 50S ribosomal protein L13 (rplM) CSB-YP393509PZJ
CSB-EP393509PZJ
CSB-BP393509PZJ
CSB-MP393509PZJ
CSB-EP393509PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP393510PZJ
CSB-EP393510PZJ
CSB-BP393510PZJ
CSB-MP393510PZJ
CSB-EP393510PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Aspartate carbamoyltransferase (pyrB) CSB-YP393511PZJ
CSB-EP393511PZJ
CSB-BP393511PZJ
CSB-MP393511PZJ
CSB-EP393511PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP393512PZJ
CSB-EP393512PZJ
CSB-BP393512PZJ
CSB-MP393512PZJ
CSB-EP393512PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis 60 kDa chaperonin (groL), partial CSB-YP393513PZJ
CSB-EP393513PZJ
CSB-BP393513PZJ
CSB-MP393513PZJ
CSB-EP393513PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP393514PZJ
CSB-EP393514PZJ
CSB-BP393514PZJ
CSB-MP393514PZJ
CSB-EP393514PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Probable rRNA maturation factor (Cvib_0701) CSB-YP393515PZJ
CSB-EP393515PZJ
CSB-BP393515PZJ
CSB-MP393515PZJ
CSB-EP393515PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis D-alanine--D-alanine ligase (ddl) CSB-YP393516PZJ
CSB-EP393516PZJ
CSB-BP393516PZJ
CSB-MP393516PZJ
CSB-EP393516PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Peptidyl-tRNA hydrolase (pth) CSB-YP393517PZJ
CSB-EP393517PZJ
CSB-BP393517PZJ
CSB-MP393517PZJ
CSB-EP393517PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Maf-like protein Cvib_0777 (Cvib_0777) CSB-YP393518PZJ
CSB-EP393518PZJ
CSB-BP393518PZJ
CSB-MP393518PZJ
CSB-EP393518PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Acetylglutamate kinase (argB) CSB-YP393519PZJ
CSB-EP393519PZJ
CSB-BP393519PZJ
CSB-MP393519PZJ
CSB-EP393519PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Arginine repressor (argR) CSB-YP393520PZJ
CSB-EP393520PZJ
CSB-BP393520PZJ
CSB-MP393520PZJ
CSB-EP393520PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris vibrioformis Thiamine-phosphate synthase (thiE) CSB-YP393521PZJ
CSB-EP393521PZJ
CSB-BP393521PZJ
CSB-MP393521PZJ
CSB-EP393521PZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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