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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas stutzeri 30S ribosomal protein S15 (rpsO) CSB-YP395244PZP
CSB-EP395244PZP
CSB-BP395244PZP
CSB-MP395244PZP
CSB-EP395244PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Phosphoglucosamine mutase (glmM) CSB-YP395245PZP
CSB-EP395245PZP
CSB-BP395245PZP
CSB-MP395245PZP
CSB-EP395245PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Pyrimidine monooxygenase RutA (rutA) CSB-YP395246PZP
CSB-EP395246PZP
CSB-BP395246PZP
CSB-MP395246PZP
CSB-EP395246PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 30S ribosomal protein S18 (rpsR) CSB-YP395247PZP
CSB-EP395247PZP
CSB-BP395247PZP
CSB-MP395247PZP
CSB-EP395247PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Urease accessory protein UreD (ureD) CSB-YP395248PZP
CSB-EP395248PZP
CSB-BP395248PZP
CSB-MP395248PZP
CSB-EP395248PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP395249PZP
CSB-EP395249PZP
CSB-BP395249PZP
CSB-MP395249PZP
CSB-EP395249PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP395250PZP
CSB-EP395250PZP
CSB-BP395250PZP
CSB-MP395250PZP
CSB-EP395250PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri S-adenosylmethionine synthase (metK) CSB-YP395251PZP
CSB-EP395251PZP
CSB-BP395251PZP
CSB-MP395251PZP
CSB-EP395251PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri tRNA (guanine-N (7)-)-methyltransferase CSB-YP395252PZP
CSB-EP395252PZP
CSB-BP395252PZP
CSB-MP395252PZP
CSB-EP395252PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP395253PZP
CSB-EP395253PZP
CSB-BP395253PZP
CSB-MP395253PZP
CSB-EP395253PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0473 protein SSU05_0068 (SSU05_0068) CSB-YP395254SUS
CSB-EP395254SUS
CSB-BP395254SUS
CSB-MP395254SUS
CSB-EP395254SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S17 (rpsQ) CSB-YP395255SUS
CSB-EP395255SUS
CSB-BP395255SUS
CSB-MP395255SUS
CSB-EP395255SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Adenylate kinase (adk) CSB-YP395256SUS
CSB-EP395256SUS
CSB-BP395256SUS
CSB-MP395256SUS
CSB-EP395256SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Phosphoglycerate kinase (pgk) CSB-YP395257SUS
CSB-EP395257SUS
CSB-BP395257SUS
CSB-MP395257SUS
CSB-EP395257SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L32 (rpmF) CSB-YP395258SUS
CSB-EP395258SUS
CSB-BP395258SUS
CSB-MP395258SUS
CSB-EP395258SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0397 protein SSU05_0404 (SSU05_0404), partial CSB-YP395259SUS1
CSB-EP395259SUS1
CSB-BP395259SUS1
CSB-MP395259SUS1
CSB-EP395259SUS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0374 protein SSU05_0445 (SSU05_0445) CSB-YP395260SUS
CSB-EP395260SUS
CSB-BP395260SUS
CSB-MP395260SUS
CSB-EP395260SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Putative sporulation transcription regulator WhiA (whiA) CSB-YP395261SUS
CSB-EP395261SUS
CSB-BP395261SUS
CSB-MP395261SUS
CSB-EP395261SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Formate--tetrahydrofolate ligase (fhs), partial CSB-YP395262SUS
CSB-EP395262SUS
CSB-BP395262SUS
CSB-MP395262SUS
CSB-EP395262SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L27 (rpmA) CSB-YP395263SUS
CSB-EP395263SUS
CSB-BP395263SUS
CSB-MP395263SUS
CSB-EP395263SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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