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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas stutzeri Cell volume regulation protein A homolog (cvrA), partial CSB-YP394904PZP
CSB-EP394904PZP
CSB-BP394904PZP
CSB-MP394904PZP
CSB-EP394904PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Aspartate carbamoyltransferase (pyrB) CSB-YP394905PZP
CSB-EP394905PZP
CSB-BP394905PZP
CSB-MP394905PZP
CSB-EP394905PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Protein-export protein secB (secB) CSB-YP394906PZP
CSB-EP394906PZP
CSB-BP394906PZP
CSB-MP394906PZP
CSB-EP394906PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Malonate decarboxylase acyl carrier protein (mdcC) CSB-YP394907PZP
CSB-EP394907PZP
CSB-BP394907PZP
CSB-MP394907PZP
CSB-EP394907PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Membrane protein insertase YidC (yidC), partial CSB-YP394908PZP1
CSB-EP394908PZP1
CSB-BP394908PZP1
CSB-MP394908PZP1
CSB-EP394908PZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP394909SUS
CSB-EP394909SUS
CSB-BP394909SUS
CSB-MP394909SUS
CSB-EP394909SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L23 (rplW) CSB-YP394910SUS
CSB-EP394910SUS
CSB-BP394910SUS
CSB-MP394910SUS
CSB-EP394910SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L5 (rplE) CSB-YP394911SUS
CSB-EP394911SUS
CSB-BP394911SUS
CSB-MP394911SUS
CSB-EP394911SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L30 (rpmD) CSB-YP394912SUS
CSB-EP394912SUS
CSB-BP394912SUS
CSB-MP394912SUS
CSB-EP394912SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S11 (rpsK) CSB-YP394913SUS
CSB-EP394913SUS
CSB-BP394913SUS
CSB-MP394913SUS
CSB-EP394913SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP394914SUS
CSB-EP394914SUS
CSB-BP394914SUS
CSB-MP394914SUS
CSB-EP394914SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L33 (rpmG) CSB-YP394915SUS
CSB-EP394915SUS
CSB-BP394915SUS
CSB-MP394915SUS
CSB-EP394915SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0340 protein SSU05_0314 (SSU05_0314) CSB-YP394916SUS
CSB-EP394916SUS
CSB-BP394916SUS
CSB-MP394916SUS
CSB-EP394916SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP394917SUS
CSB-EP394917SUS
CSB-BP394917SUS
CSB-MP394917SUS
CSB-EP394917SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0398 protein SSU05_0416 (SSU05_0416) CSB-YP394918SUS
CSB-EP394918SUS
CSB-BP394918SUS
CSB-MP394918SUS
CSB-EP394918SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP394919SUS
CSB-EP394919SUS
CSB-BP394919SUS
CSB-MP394919SUS
CSB-EP394919SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Gamma-glutamyl phosphate reductase (proA) CSB-YP394920SUS
CSB-EP394920SUS
CSB-BP394920SUS
CSB-MP394920SUS
CSB-EP394920SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis GTPase obg (obg) CSB-YP394921SUS
CSB-EP394921SUS
CSB-BP394921SUS
CSB-MP394921SUS
CSB-EP394921SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis GMP reductase (guaC) CSB-YP394922SUS
CSB-EP394922SUS
CSB-BP394922SUS
CSB-MP394922SUS
CSB-EP394922SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP394923SUS
CSB-EP394923SUS
CSB-BP394923SUS
CSB-MP394923SUS
CSB-EP394923SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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