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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas mendocina Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP394504PZM
CSB-EP394504PZM
CSB-BP394504PZM
CSB-MP394504PZM
CSB-EP394504PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Methionine--tRNA ligase (metG), partial CSB-YP394505YAL
CSB-EP394505YAL
CSB-BP394505YAL
CSB-MP394505YAL
CSB-EP394505YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Methylglyoxal synthase (mgsA) CSB-YP394506YAL
CSB-EP394506YAL
CSB-BP394506YAL
CSB-MP394506YAL
CSB-EP394506YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Autonomous glycyl radical cofactor (grcA) CSB-YP394507YAL
CSB-EP394507YAL
CSB-BP394507YAL
CSB-MP394507YAL
CSB-EP394507YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Urease accessory protein UreF (ureF) CSB-YP394508YAL
CSB-EP394508YAL
CSB-BP394508YAL
CSB-MP394508YAL
CSB-EP394508YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis [Protein-PII] uridylyltransferase (glnD), partial CSB-YP394509YAL
CSB-EP394509YAL
CSB-BP394509YAL
CSB-MP394509YAL
CSB-EP394509YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protease HtpX (htpX), partial CSB-YP394510YAL1
CSB-EP394510YAL1
CSB-BP394510YAL1
CSB-MP394510YAL1
CSB-EP394510YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis High frequency lysogenization protein HflD homolog (hflD) CSB-YP394511YAL
CSB-EP394511YAL
CSB-BP394511YAL
CSB-MP394511YAL
CSB-EP394511YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis N-methyl-L-tryptophan oxidase (solA) CSB-YP394512YAL
CSB-EP394512YAL
CSB-BP394512YAL
CSB-MP394512YAL
CSB-EP394512YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis DNA protection during starvation protein (dps) CSB-YP394513YAL
CSB-EP394513YAL
CSB-BP394513YAL
CSB-MP394513YAL
CSB-EP394513YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP394514YAL1
CSB-EP394514YAL1
CSB-BP394514YAL1
CSB-MP394514YAL1
CSB-EP394514YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP394515YAL
CSB-EP394515YAL
CSB-BP394515YAL
CSB-MP394515YAL
CSB-EP394515YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cell division protein ZipA homolog (zipA), partial CSB-YP394516YAL
CSB-EP394516YAL
CSB-BP394516YAL
CSB-MP394516YAL
CSB-EP394516YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Periplasmic nitrate reductase (napA), partial CSB-YP394517YAL
CSB-EP394517YAL
CSB-BP394517YAL
CSB-MP394517YAL
CSB-EP394517YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0756 membrane protein YPDSF_2162 (YPDSF_2162), partial CSB-YP394518YAL1
CSB-EP394518YAL1
CSB-BP394518YAL1
CSB-MP394518YAL1
CSB-EP394518YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP394519YAL
CSB-EP394519YAL
CSB-BP394519YAL
CSB-MP394519YAL
CSB-EP394519YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Co-chaperone protein hscB (hscB) CSB-YP394520YAL
CSB-EP394520YAL
CSB-BP394520YAL
CSB-MP394520YAL
CSB-EP394520YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Asparagine--tRNA ligase (asnS) CSB-YP394521YAL
CSB-EP394521YAL
CSB-BP394521YAL
CSB-MP394521YAL
CSB-EP394521YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP394522YAL
CSB-EP394522YAL
CSB-BP394522YAL
CSB-MP394522YAL
CSB-EP394522YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0352 protein YPDSF_2433 (YPDSF_2433) CSB-YP394523YAL
CSB-EP394523YAL
CSB-BP394523YAL
CSB-MP394523YAL
CSB-EP394523YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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