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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium gilvum 50S ribosomal protein L32 (rpmF) CSB-YP393722MOK
CSB-EP393722MOK
CSB-BP393722MOK
CSB-MP393722MOK
CSB-EP393722MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP393723MOK
CSB-EP393723MOK
CSB-BP393723MOK
CSB-MP393723MOK
CSB-EP393723MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP393724MOK
CSB-EP393724MOK
CSB-BP393724MOK
CSB-MP393724MOK
CSB-EP393724MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Acetaldehyde dehydrogenase (Mflv_1521) CSB-YP393725MOK
CSB-EP393725MOK
CSB-BP393725MOK
CSB-MP393725MOK
CSB-EP393725MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribonuclease PH (rph) CSB-YP393726MOK
CSB-EP393726MOK
CSB-BP393726MOK
CSB-MP393726MOK
CSB-EP393726MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP synthase gamma chain (atpG) CSB-YP393727MOK
CSB-EP393727MOK
CSB-BP393727MOK
CSB-MP393727MOK
CSB-EP393727MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP synthase subunit c (atpE), partial CSB-YP393728MOK1
CSB-EP393728MOK1
CSB-BP393728MOK1
CSB-MP393728MOK1
CSB-EP393728MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP393729MOK
CSB-EP393729MOK
CSB-BP393729MOK
CSB-MP393729MOK
CSB-EP393729MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Pantothenate kinase (coaA) CSB-YP393730MOK
CSB-EP393730MOK
CSB-BP393730MOK
CSB-MP393730MOK
CSB-EP393730MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP393731MOK
CSB-EP393731MOK
CSB-BP393731MOK
CSB-MP393731MOK
CSB-EP393731MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP393732MOK
CSB-EP393732MOK
CSB-BP393732MOK
CSB-MP393732MOK
CSB-EP393732MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP393733MOK
CSB-EP393733MOK
CSB-BP393733MOK
CSB-MP393733MOK
CSB-EP393733MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L20 (rplT) CSB-YP393734MOK
CSB-EP393734MOK
CSB-BP393734MOK
CSB-MP393734MOK
CSB-EP393734MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Acetylglutamate kinase (argB) CSB-YP393735MOK
CSB-EP393735MOK
CSB-BP393735MOK
CSB-MP393735MOK
CSB-EP393735MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum CinA-like protein (Mflv_3280) CSB-YP393736MOK
CSB-EP393736MOK
CSB-BP393736MOK
CSB-MP393736MOK
CSB-EP393736MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Sec-independent protein translocase protein TatA (tatA), partial CSB-YP393737MOK
CSB-EP393737MOK
CSB-BP393737MOK
CSB-MP393737MOK
CSB-EP393737MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP393738MOK
CSB-EP393738MOK
CSB-BP393738MOK
CSB-MP393738MOK
CSB-EP393738MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Proteasome-associated ATPase (mpa), partial CSB-YP393739MOK
CSB-EP393739MOK
CSB-BP393739MOK
CSB-MP393739MOK
CSB-EP393739MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP393740MOK
CSB-EP393740MOK
CSB-BP393740MOK
CSB-MP393740MOK
CSB-EP393740MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Undecaprenyl-diphosphatase (uppP), partial CSB-YP393741MOK
CSB-EP393741MOK
CSB-BP393741MOK
CSB-MP393741MOK
CSB-EP393741MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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