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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salinispora tropica Aspartate 1-decarboxylase (panD) CSB-YP394122STD
CSB-EP394122STD
CSB-BP394122STD
CSB-MP394122STD
CSB-EP394122STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP394123STD
CSB-EP394123STD
CSB-BP394123STD
CSB-MP394123STD
CSB-EP394123STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica UPF0042 nucleotide-binding protein Strop_3101 (Strop_3101) CSB-YP394124STD
CSB-EP394124STD
CSB-BP394124STD
CSB-MP394124STD
CSB-EP394124STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Biotin synthase (bioB) CSB-YP394125CVX
CSB-EP394125CVX
CSB-BP394125CVX
CSB-MP394125CVX
CSB-EP394125CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0145 protein Csac_0771 (Csac_0771) CSB-YP394126CVX
CSB-EP394126CVX
CSB-BP394126CVX
CSB-MP394126CVX
CSB-EP394126CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Octanoyltransferase (lipB) CSB-YP394127CVX
CSB-EP394127CVX
CSB-BP394127CVX
CSB-MP394127CVX
CSB-EP394127CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP394128CVX
CSB-EP394128CVX
CSB-BP394128CVX
CSB-MP394128CVX
CSB-EP394128CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 30S ribosomal protein S7 (rpsG) CSB-YP394129CVX
CSB-EP394129CVX
CSB-BP394129CVX
CSB-MP394129CVX
CSB-EP394129CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Homoserine kinase (thrB) CSB-YP394130CVX
CSB-EP394130CVX
CSB-BP394130CVX
CSB-MP394130CVX
CSB-EP394130CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP394131CVX
CSB-EP394131CVX
CSB-BP394131CVX
CSB-MP394131CVX
CSB-EP394131CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP394132CVX
CSB-EP394132CVX
CSB-BP394132CVX
CSB-MP394132CVX
CSB-EP394132CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Probable phosphatase Csac_1188 (Csac_1188) CSB-YP394133CVX
CSB-EP394133CVX
CSB-BP394133CVX
CSB-MP394133CVX
CSB-EP394133CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Redox-sensing transcriptional repressor rex (rex) CSB-YP394134CVX
CSB-EP394134CVX
CSB-BP394134CVX
CSB-MP394134CVX
CSB-EP394134CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 30S ribosomal protein S9 (rpsI) CSB-YP394135CVX
CSB-EP394135CVX
CSB-BP394135CVX
CSB-MP394135CVX
CSB-EP394135CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L31 (rpmE) CSB-YP394136CVX
CSB-EP394136CVX
CSB-BP394136CVX
CSB-MP394136CVX
CSB-EP394136CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP394137CVX
CSB-EP394137CVX
CSB-BP394137CVX
CSB-MP394137CVX
CSB-EP394137CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L21 (rplU) CSB-YP394138CVX
CSB-EP394138CVX
CSB-BP394138CVX
CSB-MP394138CVX
CSB-EP394138CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus UPF0133 protein Csac_1593 (Csac_1593) CSB-YP394139CVX
CSB-EP394139CVX
CSB-BP394139CVX
CSB-MP394139CVX
CSB-EP394139CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP394140CVX
CSB-EP394140CVX
CSB-BP394140CVX
CSB-MP394140CVX
CSB-EP394140CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Gamma-glutamyl phosphate reductase (proA) CSB-YP394141CVX
CSB-EP394141CVX
CSB-BP394141CVX
CSB-MP394141CVX
CSB-EP394141CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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