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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter sp. L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP394323EIZ
CSB-EP394323EIZ
CSB-BP394323EIZ
CSB-MP394323EIZ
CSB-EP394323EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP394324EIZ
CSB-EP394324EIZ
CSB-BP394324EIZ
CSB-MP394324EIZ
CSB-EP394324EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Regulator of sigma D (rsd) CSB-YP394325EIZ
CSB-EP394325EIZ
CSB-BP394325EIZ
CSB-MP394325EIZ
CSB-EP394325EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein psiE homolog (psiE), partial CSB-YP394326EIZ1
CSB-EP394326EIZ1
CSB-BP394326EIZ1
CSB-MP394326EIZ1
CSB-EP394326EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Fumarate reductase subunit D (frdD), partial CSB-YP394327EIZ1
CSB-EP394327EIZ1
CSB-BP394327EIZ1
CSB-MP394327EIZ1
CSB-EP394327EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Adenylosuccinate synthetase (purA) CSB-YP394328EIZ
CSB-EP394328EIZ
CSB-BP394328EIZ
CSB-MP394328EIZ
CSB-EP394328EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP394329EIZ
CSB-EP394329EIZ
CSB-BP394329EIZ
CSB-MP394329EIZ
CSB-EP394329EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP394330EIZ
CSB-EP394330EIZ
CSB-BP394330EIZ
CSB-MP394330EIZ
CSB-EP394330EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Na (+)/H (+) antiporter nhaA, partial CSB-YP394331EIZ
CSB-EP394331EIZ
CSB-BP394331EIZ
CSB-MP394331EIZ
CSB-EP394331EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP394332EIZ
CSB-EP394332EIZ
CSB-BP394332EIZ
CSB-MP394332EIZ
CSB-EP394332EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP394333EIZ
CSB-EP394333EIZ
CSB-BP394333EIZ
CSB-MP394333EIZ
CSB-EP394333EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0231 protein Ent638_0667 (Ent638_0667) CSB-YP394334EIZ
CSB-EP394334EIZ
CSB-BP394334EIZ
CSB-MP394334EIZ
CSB-EP394334EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP394335EIZ
CSB-EP394335EIZ
CSB-BP394335EIZ
CSB-MP394335EIZ
CSB-EP394335EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0325 protein Ent638_0703 (Ent638_0703) CSB-YP394336EIZ
CSB-EP394336EIZ
CSB-BP394336EIZ
CSB-MP394336EIZ
CSB-EP394336EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Lipid-A-disaccharide synthase (lpxB) CSB-YP394337EIZ
CSB-EP394337EIZ
CSB-BP394337EIZ
CSB-MP394337EIZ
CSB-EP394337EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Xanthine phosphoribosyltransferase (gpt) CSB-YP394338EIZ
CSB-EP394338EIZ
CSB-BP394338EIZ
CSB-MP394338EIZ
CSB-EP394338EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Recombination-associated protein rdgC (rdgC) CSB-YP394339EIZ
CSB-EP394339EIZ
CSB-BP394339EIZ
CSB-MP394339EIZ
CSB-EP394339EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 7-cyano-7-deazaguanine synthase (queC) CSB-YP394340EIZ
CSB-EP394340EIZ
CSB-BP394340EIZ
CSB-MP394340EIZ
CSB-EP394340EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0133 protein Ent638_0951 (Ent638_0951) CSB-YP394341EIZ
CSB-EP394341EIZ
CSB-BP394341EIZ
CSB-MP394341EIZ
CSB-EP394341EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0250 protein Ent638_1166 (Ent638_1166) CSB-YP394342EIZ
CSB-EP394342EIZ
CSB-BP394342EIZ
CSB-MP394342EIZ
CSB-EP394342EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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