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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium gilvum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP393742MOK
CSB-EP393742MOK
CSB-BP393742MOK
CSB-MP393742MOK
CSB-EP393742MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP393743MOK
CSB-EP393743MOK
CSB-BP393743MOK
CSB-MP393743MOK
CSB-EP393743MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP393744MOK
CSB-EP393744MOK
CSB-BP393744MOK
CSB-MP393744MOK
CSB-EP393744MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Elongation factor P (efp) CSB-YP393745MOK
CSB-EP393745MOK
CSB-BP393745MOK
CSB-MP393745MOK
CSB-EP393745MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Aspartate carbamoyltransferase (pyrB) CSB-YP393746MOK
CSB-EP393746MOK
CSB-BP393746MOK
CSB-MP393746MOK
CSB-EP393746MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UvrABC system protein C (uvrC), partial CSB-YP393747MOK
CSB-EP393747MOK
CSB-BP393747MOK
CSB-MP393747MOK
CSB-EP393747MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum UPF0042 nucleotide-binding protein Mflv_3714 (Mflv_3714) CSB-YP393748MOK
CSB-EP393748MOK
CSB-BP393748MOK
CSB-MP393748MOK
CSB-EP393748MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Protein RecA (recA) CSB-YP393749MOK
CSB-EP393749MOK
CSB-BP393749MOK
CSB-MP393749MOK
CSB-EP393749MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Transcriptional repressor NrdR (nrdR) CSB-YP393750MOK
CSB-EP393750MOK
CSB-BP393750MOK
CSB-MP393750MOK
CSB-EP393750MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Na (+)/H (+) antiporter nhaA 2, partial CSB-YP393751MOK
CSB-EP393751MOK
CSB-BP393751MOK
CSB-MP393751MOK
CSB-EP393751MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP393752MOK
CSB-EP393752MOK
CSB-BP393752MOK
CSB-MP393752MOK
CSB-EP393752MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum SsrA-binding protein (smpB) CSB-YP393753MOK
CSB-EP393753MOK
CSB-BP393753MOK
CSB-MP393753MOK
CSB-EP393753MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP393754MOK
CSB-EP393754MOK
CSB-BP393754MOK
CSB-MP393754MOK
CSB-EP393754MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP393755MOK
CSB-EP393755MOK
CSB-BP393755MOK
CSB-MP393755MOK
CSB-EP393755MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Polyphosphate kinase (ppk), partial CSB-YP393756MOK
CSB-EP393756MOK
CSB-BP393756MOK
CSB-MP393756MOK
CSB-EP393756MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Uncharacterized oxidoreductase Mflv_4205 (Mflv_4205) CSB-YP393757MOK
CSB-EP393757MOK
CSB-BP393757MOK
CSB-MP393757MOK
CSB-EP393757MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP393758MOK
CSB-EP393758MOK
CSB-BP393758MOK
CSB-MP393758MOK
CSB-EP393758MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Ribosome maturation factor RimM (rimM) CSB-YP393759MOK
CSB-EP393759MOK
CSB-BP393759MOK
CSB-MP393759MOK
CSB-EP393759MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Tyrosine recombinase XerC (xerC) CSB-YP393760MOK
CSB-EP393760MOK
CSB-BP393760MOK
CSB-MP393760MOK
CSB-EP393760MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum 50S ribosomal protein L22 (rplV) CSB-YP393761MOK
CSB-EP393761MOK
CSB-BP393761MOK
CSB-MP393761MOK
CSB-EP393761MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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