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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis GTPase obg (obg) CSB-YP393862YAL
CSB-EP393862YAL
CSB-BP393862YAL
CSB-MP393862YAL
CSB-EP393862YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP393863YAL
CSB-EP393863YAL
CSB-BP393863YAL
CSB-MP393863YAL
CSB-EP393863YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein hfq (hfq) CSB-YP393864YAL
CSB-EP393864YAL
CSB-BP393864YAL
CSB-MP393864YAL
CSB-EP393864YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Elongation factor P (efp) CSB-YP393865YAL
CSB-EP393865YAL
CSB-BP393865YAL
CSB-MP393865YAL
CSB-EP393865YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis L-rhamnose mutarotase (rhaM) CSB-YP393866YAL
CSB-EP393866YAL
CSB-BP393866YAL
CSB-MP393866YAL
CSB-EP393866YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 50S ribosomal protein L1 (rplA) CSB-YP393867YAL
CSB-EP393867YAL
CSB-BP393867YAL
CSB-MP393867YAL
CSB-EP393867YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cell division protein ZapB (zapB) CSB-YP393868YAL
CSB-EP393868YAL
CSB-BP393868YAL
CSB-MP393868YAL
CSB-EP393868YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP393869YAL
CSB-EP393869YAL
CSB-BP393869YAL
CSB-MP393869YAL
CSB-EP393869YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis DNA ligase B (ligB), partial CSB-YP393870YAL
CSB-EP393870YAL
CSB-BP393870YAL
CSB-MP393870YAL
CSB-EP393870YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis D-ribose pyranase (rbsD) CSB-YP393871YAL
CSB-EP393871YAL
CSB-BP393871YAL
CSB-MP393871YAL
CSB-EP393871YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA modification GTPase MnmE (mnmE) CSB-YP393872YAL
CSB-EP393872YAL
CSB-BP393872YAL
CSB-MP393872YAL
CSB-EP393872YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP393873ILR
CSB-EP393873ILR
CSB-BP393873ILR
CSB-MP393873ILR
CSB-EP393873ILR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP393874ILR
CSB-EP393874ILR
CSB-BP393874ILR
CSB-MP393874ILR
CSB-EP393874ILR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP393875ILQ
CSB-EP393875ILQ
CSB-BP393875ILQ
CSB-MP393875ILQ
CSB-EP393875ILQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European bat lyssavirus 1 RNA-directed RNA polymerase L (L), partial CSB-YP393876EJH
CSB-EP393876EJH
CSB-BP393876EJH
CSB-MP393876EJH
CSB-EP393876EJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Vitelline membrane protein Vm32E (Vm32E) CSB-YP393877DMJ
CSB-EP393877DMJ
CSB-BP393877DMJ
CSB-MP393877DMJ
CSB-EP393877DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Sec-independent protein translocase protein TatB (tatB), partial CSB-YP393878PZP
CSB-EP393878PZP
CSB-BP393878PZP
CSB-MP393878PZP
CSB-EP393878PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Acetylglutamate kinase (argB) CSB-YP393879PZP
CSB-EP393879PZP
CSB-BP393879PZP
CSB-MP393879PZP
CSB-EP393879PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Disulfide bond formation protein B (dsbB), partial CSB-YP393880PZP1
CSB-EP393880PZP1
CSB-BP393880PZP1
CSB-MP393880PZP1
CSB-EP393880PZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Anthranilate phosphoribosyltransferase (trpD) CSB-YP393881PZP
CSB-EP393881PZP
CSB-BP393881PZP
CSB-MP393881PZP
CSB-EP393881PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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