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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis Urease accessory protein UreE (ureE) CSB-YP393822YAL
CSB-EP393822YAL
CSB-BP393822YAL
CSB-MP393822YAL
CSB-EP393822YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Proline--tRNA ligase (proS), partial CSB-YP393823YAL
CSB-EP393823YAL
CSB-BP393823YAL
CSB-MP393823YAL
CSB-EP393823YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP393824YAL
CSB-EP393824YAL
CSB-BP393824YAL
CSB-MP393824YAL
CSB-EP393824YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP393825YAL
CSB-EP393825YAL
CSB-BP393825YAL
CSB-MP393825YAL
CSB-EP393825YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP393826YAL1
CSB-EP393826YAL1
CSB-BP393826YAL1
CSB-MP393826YAL1
CSB-EP393826YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 50S ribosomal protein L32 (rpmF) CSB-YP393827YAL
CSB-EP393827YAL
CSB-BP393827YAL
CSB-MP393827YAL
CSB-EP393827YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP393828YAL
CSB-EP393828YAL
CSB-BP393828YAL
CSB-MP393828YAL
CSB-EP393828YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP393829YAL1
CSB-EP393829YAL1
CSB-BP393829YAL1
CSB-MP393829YAL1
CSB-EP393829YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Chorismate synthase (aroC) CSB-YP393830YAL
CSB-EP393830YAL
CSB-BP393830YAL
CSB-MP393830YAL
CSB-EP393830YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis N-acetylmannosamine kinase (nanK) CSB-YP393831YAL
CSB-EP393831YAL
CSB-BP393831YAL
CSB-MP393831YAL
CSB-EP393831YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP393832YAL
CSB-EP393832YAL
CSB-BP393832YAL
CSB-MP393832YAL
CSB-EP393832YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Dihydrodipicolinate synthase (dapA) CSB-YP393833YAL
CSB-EP393833YAL
CSB-BP393833YAL
CSB-MP393833YAL
CSB-EP393833YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP393834YAL
CSB-EP393834YAL
CSB-BP393834YAL
CSB-MP393834YAL
CSB-EP393834YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP393835YAL
CSB-EP393835YAL
CSB-BP393835YAL
CSB-MP393835YAL
CSB-EP393835YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP393836YAL
CSB-EP393836YAL
CSB-BP393836YAL
CSB-MP393836YAL
CSB-EP393836YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Integration host factor subunit beta (ihfB) CSB-YP393837YAL
CSB-EP393837YAL
CSB-BP393837YAL
CSB-MP393837YAL
CSB-EP393837YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP393838YAL
CSB-EP393838YAL
CSB-BP393838YAL
CSB-MP393838YAL
CSB-EP393838YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Nucleoid-associated protein YPDSF_2432 (YPDSF_2432) CSB-YP393839YAL
CSB-EP393839YAL
CSB-BP393839YAL
CSB-MP393839YAL
CSB-EP393839YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UvrABC system protein B (uvrB), partial CSB-YP393840YAL
CSB-EP393840YAL
CSB-BP393840YAL
CSB-MP393840YAL
CSB-EP393840YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Glutamine--tRNA ligase (glnS), partial CSB-YP393841YAL
CSB-EP393841YAL
CSB-BP393841YAL
CSB-MP393841YAL
CSB-EP393841YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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