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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum arsenaticum DNA repair and recombination protein radA (radA) CSB-YP394062PZU
CSB-EP394062PZU
CSB-BP394062PZU
CSB-MP394062PZU
CSB-EP394062PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S12P (rps12p) CSB-YP394063PZU
CSB-EP394063PZU
CSB-BP394063PZU
CSB-MP394063PZU
CSB-EP394063PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP394064RLG
CSB-EP394064RLG
CSB-BP394064RLG
CSB-MP394064RLG
CSB-EP394064RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides (3S)-malyl-CoA thioesterase CSB-YP394065RLG
CSB-EP394065RLG
CSB-BP394065RLG
CSB-MP394065RLG
CSB-EP394065RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP394066RLG1
CSB-EP394066RLG1
CSB-BP394066RLG1
CSB-MP394066RLG1
CSB-EP394066RLG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Tryptophan synthase alpha chain (trpA) CSB-YP394067RLG
CSB-EP394067RLG
CSB-BP394067RLG
CSB-MP394067RLG
CSB-EP394067RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Aspartate--tRNA ligase (aspS), partial CSB-YP394068RLG
CSB-EP394068RLG
CSB-BP394068RLG
CSB-MP394068RLG
CSB-EP394068RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Formate--tetrahydrofolate ligase (fhs), partial CSB-YP394069RLG
CSB-EP394069RLG
CSB-BP394069RLG
CSB-MP394069RLG
CSB-EP394069RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Octanoyltransferase (lipB) CSB-YP394070RLG
CSB-EP394070RLG
CSB-BP394070RLG
CSB-MP394070RLG
CSB-EP394070RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP394071RLG
CSB-EP394071RLG
CSB-BP394071RLG
CSB-MP394071RLG
CSB-EP394071RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glutamate racemase (murI) CSB-YP394072RLG
CSB-EP394072RLG
CSB-BP394072RLG
CSB-MP394072RLG
CSB-EP394072RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Alanine racemase (alr) CSB-YP394073RLG
CSB-EP394073RLG
CSB-BP394073RLG
CSB-MP394073RLG
CSB-EP394073RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP394074RLG
CSB-EP394074RLG
CSB-BP394074RLG
CSB-MP394074RLG
CSB-EP394074RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable transaldolase (tal) CSB-YP394075RLG
CSB-EP394075RLG
CSB-BP394075RLG
CSB-MP394075RLG
CSB-EP394075RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP394076RLG
CSB-EP394076RLG
CSB-BP394076RLG
CSB-MP394076RLG
CSB-EP394076RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L9 (rplI) CSB-YP394077RLG
CSB-EP394077RLG
CSB-BP394077RLG
CSB-MP394077RLG
CSB-EP394077RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP394078RLG
CSB-EP394078RLG
CSB-BP394078RLG
CSB-MP394078RLG
CSB-EP394078RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-dehydroquinate synthase (aroB) CSB-YP394079RLG
CSB-EP394079RLG
CSB-BP394079RLG
CSB-MP394079RLG
CSB-EP394079RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP394080RLG
CSB-EP394080RLG
CSB-BP394080RLG
CSB-MP394080RLG
CSB-EP394080RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L32 (rpmF) CSB-YP394081RLG
CSB-EP394081RLG
CSB-BP394081RLG
CSB-MP394081RLG
CSB-EP394081RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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