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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus suis Ribosome maturation factor RimM (rimM) CSB-YP395264SUS
CSB-EP395264SUS
CSB-BP395264SUS
CSB-MP395264SUS
CSB-EP395264SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP395265SUS
CSB-EP395265SUS
CSB-BP395265SUS
CSB-MP395265SUS
CSB-EP395265SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Alanine--tRNA ligase (alaS), partial CSB-YP395266SUS
CSB-EP395266SUS
CSB-BP395266SUS
CSB-MP395266SUS
CSB-EP395266SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Ribonuclease Z (rnz) CSB-YP395267SUS
CSB-EP395267SUS
CSB-BP395267SUS
CSB-MP395267SUS
CSB-EP395267SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Recombination protein RecR (recR) CSB-YP395268SUS
CSB-EP395268SUS
CSB-BP395268SUS
CSB-MP395268SUS
CSB-EP395268SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP395269SUS
CSB-EP395269SUS
CSB-BP395269SUS
CSB-MP395269SUS
CSB-EP395269SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Phosphoglucosamine mutase (glmM) CSB-YP395270SUS
CSB-EP395270SUS
CSB-BP395270SUS
CSB-MP395270SUS
CSB-EP395270SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis NH (3)-dependent NAD (+) synthetase CSB-YP395271SUS
CSB-EP395271SUS
CSB-BP395271SUS
CSB-MP395271SUS
CSB-EP395271SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP395272SUS
CSB-EP395272SUS
CSB-BP395272SUS
CSB-MP395272SUS
CSB-EP395272SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Protein translocase subunit SecA (secA), partial CSB-YP395273SUS
CSB-EP395273SUS
CSB-BP395273SUS
CSB-MP395273SUS
CSB-EP395273SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP395274SUS
CSB-EP395274SUS
CSB-BP395274SUS
CSB-MP395274SUS
CSB-EP395274SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Aspartate--tRNA ligase (aspS), partial CSB-YP395275SUS
CSB-EP395275SUS
CSB-BP395275SUS
CSB-MP395275SUS
CSB-EP395275SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0297 protein SSU98_0066 (SSU98_0066) CSB-YP395276SUT
CSB-EP395276SUT
CSB-BP395276SUT
CSB-MP395276SUT
CSB-EP395276SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L2 (rplB) CSB-YP395277SUT
CSB-EP395277SUT
CSB-BP395277SUT
CSB-MP395277SUT
CSB-EP395277SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L29 (rpmC) CSB-YP395278SUT
CSB-EP395278SUT
CSB-BP395278SUT
CSB-MP395278SUT
CSB-EP395278SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Tyrosine--tRNA ligase (tyrS) CSB-YP395279SUT
CSB-EP395279SUT
CSB-BP395279SUT
CSB-MP395279SUT
CSB-EP395279SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S7 (rpsG) CSB-YP395280SUT
CSB-EP395280SUT
CSB-BP395280SUT
CSB-MP395280SUT
CSB-EP395280SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0210 protein SSU98_0292 (SSU98_0292) CSB-YP395281SUT
CSB-EP395281SUT
CSB-BP395281SUT
CSB-MP395281SUT
CSB-EP395281SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP395282SUT
CSB-EP395282SUT
CSB-BP395282SUT
CSB-MP395282SUT
CSB-EP395282SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis UPF0398 protein SSU98_0402 (SSU98_0402) CSB-YP395283SUT
CSB-EP395283SUT
CSB-BP395283SUT
CSB-MP395283SUT
CSB-EP395283SUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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