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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. Glutamate 5-kinase (proB) CSB-YP393982EIZ
CSB-EP393982EIZ
CSB-BP393982EIZ
CSB-MP393982EIZ
CSB-EP393982EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Transcriptional repressor NrdR (nrdR) CSB-YP393983EIZ
CSB-EP393983EIZ
CSB-BP393983EIZ
CSB-MP393983EIZ
CSB-EP393983EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein CrcB homolog (crcB), partial CSB-YP393984EIZ1
CSB-EP393984EIZ1
CSB-BP393984EIZ1
CSB-MP393984EIZ1
CSB-EP393984EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Probable rRNA maturation factor (Ent638_1185) CSB-YP393985EIZ
CSB-EP393985EIZ
CSB-BP393985EIZ
CSB-MP393985EIZ
CSB-EP393985EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Potassium-transporting ATPase B chain (kdpB), partial CSB-YP393986EIZ1
CSB-EP393986EIZ1
CSB-BP393986EIZ1
CSB-MP393986EIZ1
CSB-EP393986EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein TolB (tolB) CSB-YP393987EIZ
CSB-EP393987EIZ
CSB-BP393987EIZ
CSB-MP393987EIZ
CSB-EP393987EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Outer-membrane lipoprotein carrier protein (lolA) CSB-YP393988EIZ
CSB-EP393988EIZ
CSB-BP393988EIZ
CSB-MP393988EIZ
CSB-EP393988EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP393989EIZ
CSB-EP393989EIZ
CSB-BP393989EIZ
CSB-MP393989EIZ
CSB-EP393989EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Alkanesulfonate monooxygenase (ssuD) CSB-YP393990EIZ
CSB-EP393990EIZ
CSB-BP393990EIZ
CSB-MP393990EIZ
CSB-EP393990EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0319 protein Ent638_1476 (Ent638_1476) CSB-YP393991EIZ
CSB-EP393991EIZ
CSB-BP393991EIZ
CSB-MP393991EIZ
CSB-EP393991EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. FMN reductase (NADH) RutF CSB-YP393992EIZ
CSB-EP393992EIZ
CSB-BP393992EIZ
CSB-MP393992EIZ
CSB-EP393992EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Glucans biosynthesis glucosyltransferase H (mdoH), partial CSB-YP393993EIZ
CSB-EP393993EIZ
CSB-BP393993EIZ
CSB-MP393993EIZ
CSB-EP393993EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0312 protein Ent638_1570 (Ent638_1570) CSB-YP393994EIZ
CSB-EP393994EIZ
CSB-BP393994EIZ
CSB-MP393994EIZ
CSB-EP393994EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L32 (rpmF) CSB-YP393995EIZ
CSB-EP393995EIZ
CSB-BP393995EIZ
CSB-MP393995EIZ
CSB-EP393995EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0229 protein Ent638_1670 (Ent638_1670) CSB-YP393996EIZ
CSB-EP393996EIZ
CSB-BP393996EIZ
CSB-MP393996EIZ
CSB-EP393996EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Integration host factor subunit alpha (ihfA) CSB-YP393997EIZ
CSB-EP393997EIZ
CSB-BP393997EIZ
CSB-MP393997EIZ
CSB-EP393997EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Cysteine desulfuration protein sufE (sufE) CSB-YP393998EIZ
CSB-EP393998EIZ
CSB-BP393998EIZ
CSB-MP393998EIZ
CSB-EP393998EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. DNA replication terminus site-binding protein (tus) CSB-YP393999EIZ
CSB-EP393999EIZ
CSB-BP393999EIZ
CSB-MP393999EIZ
CSB-EP393999EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP394000EIZ1
CSB-EP394000EIZ1
CSB-BP394000EIZ1
CSB-MP394000EIZ1
CSB-EP394000EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP394001EIZ
CSB-EP394001EIZ
CSB-BP394001EIZ
CSB-MP394001EIZ
CSB-EP394001EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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