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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas stutzeri Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP395584PZP
CSB-EP395584PZP
CSB-BP395584PZP
CSB-MP395584PZP
CSB-EP395584PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Histidine--tRNA ligase (hisS) CSB-YP395585PZP
CSB-EP395585PZP
CSB-BP395585PZP
CSB-MP395585PZP
CSB-EP395585PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Outer-membrane lipoprotein lolB (lolB) CSB-YP395586PZP
CSB-EP395586PZP
CSB-BP395586PZP
CSB-MP395586PZP
CSB-EP395586PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP395587PZP
CSB-EP395587PZP
CSB-BP395587PZP
CSB-MP395587PZP
CSB-EP395587PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Chaperone protein DnaK (dnaK), partial CSB-YP395588PZP
CSB-EP395588PZP
CSB-BP395588PZP
CSB-MP395588PZP
CSB-EP395588PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Putative NADH dehydrogenase/NAD (P)H nitroreductase PST_3601 CSB-YP395589PZP
CSB-EP395589PZP
CSB-BP395589PZP
CSB-MP395589PZP
CSB-EP395589PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Alanine racemase (alr) CSB-YP395590PZP
CSB-EP395590PZP
CSB-BP395590PZP
CSB-MP395590PZP
CSB-EP395590PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Urease subunit gamma (ureA) CSB-YP395591PZP
CSB-EP395591PZP
CSB-BP395591PZP
CSB-MP395591PZP
CSB-EP395591PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Bifunctional protein HldE (hldE) CSB-YP395592PZP
CSB-EP395592PZP
CSB-BP395592PZP
CSB-MP395592PZP
CSB-EP395592PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0061 protein PST_3881 (PST_3881) CSB-YP395593PZP
CSB-EP395593PZP
CSB-BP395593PZP
CSB-MP395593PZP
CSB-EP395593PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0301 protein PST_3956 (PST_3956) CSB-YP395594PZP
CSB-EP395594PZP
CSB-BP395594PZP
CSB-MP395594PZP
CSB-EP395594PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Dihydroxy-acid dehydratase (ilvD), partial CSB-YP395595PZP
CSB-EP395595PZP
CSB-BP395595PZP
CSB-MP395595PZP
CSB-EP395595PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Putative membrane protein insertion efficiency factor (PST_4212) CSB-YP395596PZP
CSB-EP395596PZP
CSB-BP395596PZP
CSB-MP395596PZP
CSB-EP395596PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Phosphate acyltransferase (plsX) CSB-YP395597SUS
CSB-EP395597SUS
CSB-BP395597SUS
CSB-MP395597SUS
CSB-EP395597SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L3 (rplC) CSB-YP395598SUS
CSB-EP395598SUS
CSB-BP395598SUS
CSB-MP395598SUS
CSB-EP395598SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 30S ribosomal protein S19 (rpsS) CSB-YP395599SUS
CSB-EP395599SUS
CSB-BP395599SUS
CSB-MP395599SUS
CSB-EP395599SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L14 (rplN) CSB-YP395600SUS
CSB-EP395600SUS
CSB-BP395600SUS
CSB-MP395600SUS
CSB-EP395600SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis 50S ribosomal protein L6 (rplF) CSB-YP395601SUS
CSB-EP395601SUS
CSB-BP395601SUS
CSB-MP395601SUS
CSB-EP395601SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP395602SUS
CSB-EP395602SUS
CSB-BP395602SUS
CSB-MP395602SUS
CSB-EP395602SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus suis Histidine--tRNA ligase (hisS) CSB-YP395603SUS
CSB-EP395603SUS
CSB-BP395603SUS
CSB-MP395603SUS
CSB-EP395603SUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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