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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. Autoinducer 2 kinase LsrK (lsrK), partial CSB-YP394383EIZ
CSB-EP394383EIZ
CSB-BP394383EIZ
CSB-MP394383EIZ
CSB-EP394383EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. D-tagatose-1,6-bisphosphate aldolase subunit KbaY (kbaY) CSB-YP394384EIZ
CSB-EP394384EIZ
CSB-BP394384EIZ
CSB-MP394384EIZ
CSB-EP394384EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP394385EIZ
CSB-EP394385EIZ
CSB-BP394385EIZ
CSB-MP394385EIZ
CSB-EP394385EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0042 nucleotide-binding protein Ent638_3641 (Ent638_3641) CSB-YP394386EIZ
CSB-EP394386EIZ
CSB-BP394386EIZ
CSB-MP394386EIZ
CSB-EP394386EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP394387EIZ1
CSB-EP394387EIZ1
CSB-BP394387EIZ1
CSB-MP394387EIZ1
CSB-EP394387EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Ribosomal protein L11 methyltransferase (prmA) CSB-YP394388EIZ
CSB-EP394388EIZ
CSB-BP394388EIZ
CSB-MP394388EIZ
CSB-EP394388EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Large-conductance mechanosensitive channel (mscL), partial CSB-YP394389EIZ1
CSB-EP394389EIZ1
CSB-BP394389EIZ1
CSB-MP394389EIZ1
CSB-EP394389EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L2 (rplB) CSB-YP394390EIZ
CSB-EP394390EIZ
CSB-BP394390EIZ
CSB-MP394390EIZ
CSB-EP394390EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Glutathione-regulated potassium-efflux system protein KefB (kefB), partial CSB-YP394391EIZ
CSB-EP394391EIZ
CSB-BP394391EIZ
CSB-MP394391EIZ
CSB-EP394391EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP394392EIZ
CSB-EP394392EIZ
CSB-BP394392EIZ
CSB-MP394392EIZ
CSB-EP394392EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Sulfurtransferase TusA (tusA) CSB-YP394393EIZ
CSB-EP394393EIZ
CSB-BP394393EIZ
CSB-MP394393EIZ
CSB-EP394393EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein CyaY (cyaY) CSB-YP394394EIZ
CSB-EP394394EIZ
CSB-BP394394EIZ
CSB-MP394394EIZ
CSB-EP394394EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP394395EIZ
CSB-EP394395EIZ
CSB-BP394395EIZ
CSB-MP394395EIZ
CSB-EP394395EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. HTH-type transcriptional activator rhaR (rhaR) CSB-YP394396EIZ
CSB-EP394396EIZ
CSB-BP394396EIZ
CSB-MP394396EIZ
CSB-EP394396EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. L-rhamnose mutarotase (rhaM) CSB-YP394397EIZ
CSB-EP394397EIZ
CSB-BP394397EIZ
CSB-MP394397EIZ
CSB-EP394397EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein viaA (viaA) CSB-YP394398EIZ
CSB-EP394398EIZ
CSB-BP394398EIZ
CSB-MP394398EIZ
CSB-EP394398EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Bifunctional protein GlmU (glmU) CSB-YP394399EIZ
CSB-EP394399EIZ
CSB-BP394399EIZ
CSB-MP394399EIZ
CSB-EP394399EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S2P (rps2p) CSB-YP394400PZU
CSB-EP394400PZU
CSB-BP394400PZU
CSB-MP394400PZU
CSB-EP394400PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP394401PZU
CSB-EP394401PZU
CSB-BP394401PZU
CSB-MP394401PZU
CSB-EP394401PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Deoxyribose-phosphate aldolase (deoC) CSB-YP394402PZU
CSB-EP394402PZU
CSB-BP394402PZU
CSB-MP394402PZU
CSB-EP394402PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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