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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis Chaperone protein DnaJ (dnaJ) CSB-YP395864YAL
CSB-EP395864YAL
CSB-BP395864YAL
CSB-MP395864YAL
CSB-EP395864YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Non-canonical purine NTP phosphatase (YPDSF_3180) CSB-YP395865YAL
CSB-EP395865YAL
CSB-BP395865YAL
CSB-MP395865YAL
CSB-EP395865YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP395866YAL
CSB-EP395866YAL
CSB-BP395866YAL
CSB-MP395866YAL
CSB-EP395866YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Autoinducer 2-degrading protein lsrG (lsrG) CSB-YP395867YAL
CSB-EP395867YAL
CSB-BP395867YAL
CSB-MP395867YAL
CSB-EP395867YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP395868YAL
CSB-EP395868YAL
CSB-BP395868YAL
CSB-MP395868YAL
CSB-EP395868YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP395869YAL
CSB-EP395869YAL
CSB-BP395869YAL
CSB-MP395869YAL
CSB-EP395869YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP395870YAL
CSB-EP395870YAL
CSB-BP395870YAL
CSB-MP395870YAL
CSB-EP395870YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Adenylosuccinate synthetase (purA) CSB-YP395871YAL
CSB-EP395871YAL
CSB-BP395871YAL
CSB-MP395871YAL
CSB-EP395871YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Uncharacterized protein YjeA (yjeA) CSB-YP395872YAL
CSB-EP395872YAL
CSB-BP395872YAL
CSB-MP395872YAL
CSB-EP395872YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Rhamnulokinase (rhaB) CSB-YP395873YAL
CSB-EP395873YAL
CSB-BP395873YAL
CSB-MP395873YAL
CSB-EP395873YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP395874YAL
CSB-EP395874YAL
CSB-BP395874YAL
CSB-MP395874YAL
CSB-EP395874YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP395875YAL
CSB-EP395875YAL
CSB-BP395875YAL
CSB-MP395875YAL
CSB-EP395875YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP395876YAL
CSB-EP395876YAL
CSB-BP395876YAL
CSB-MP395876YAL
CSB-EP395876YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Met repressor (metJ) CSB-YP395877YAL
CSB-EP395877YAL
CSB-BP395877YAL
CSB-MP395877YAL
CSB-EP395877YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP395878YAL
CSB-EP395878YAL
CSB-BP395878YAL
CSB-MP395878YAL
CSB-EP395878YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Low affinity potassium transport system protein kup (kup), partial CSB-YP395879YAL
CSB-EP395879YAL
CSB-BP395879YAL
CSB-MP395879YAL
CSB-EP395879YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA) CSB-YP395880ILR
CSB-EP395880ILR
CSB-BP395880ILR
CSB-MP395880ILR
CSB-EP395880ILR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M2), partial CSB-YP395881ILQ1
CSB-EP395881ILQ1
CSB-BP395881ILQ1
CSB-MP395881ILQ1
CSB-EP395881ILQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP395882ILQ
CSB-EP395882ILQ
CSB-BP395882ILQ
CSB-MP395882ILQ
CSB-EP395882ILQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European bat lyssavirus 1 Nucleoprotein (N) CSB-YP395883EJH
CSB-EP395883EJH
CSB-BP395883EJH
CSB-MP395883EJH
CSB-EP395883EJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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