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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis Potassium-transporting ATPase B chain (kdpB), partial CSB-YP394162YAL1
CSB-EP394162YAL1
CSB-BP394162YAL1
CSB-MP394162YAL1
CSB-EP394162YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0253 protein YPDSF_1647 (YPDSF_1647) CSB-YP394163YAL
CSB-EP394163YAL
CSB-BP394163YAL
CSB-MP394163YAL
CSB-EP394163YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein syd (syd) CSB-YP394164YAL
CSB-EP394164YAL
CSB-BP394164YAL
CSB-MP394164YAL
CSB-EP394164YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein Xni (xni) CSB-YP394165YAL
CSB-EP394165YAL
CSB-BP394165YAL
CSB-MP394165YAL
CSB-EP394165YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ProP effector (proQ) CSB-YP394166YAL
CSB-EP394166YAL
CSB-BP394166YAL
CSB-MP394166YAL
CSB-EP394166YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Peptidase T (pepT) CSB-YP394167YAL
CSB-EP394167YAL
CSB-BP394167YAL
CSB-MP394167YAL
CSB-EP394167YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP394168YAL
CSB-EP394168YAL
CSB-BP394168YAL
CSB-MP394168YAL
CSB-EP394168YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0176 protein YPDSF_1866 (YPDSF_1866) CSB-YP394169YAL
CSB-EP394169YAL
CSB-BP394169YAL
CSB-MP394169YAL
CSB-EP394169YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP394170YAL
CSB-EP394170YAL
CSB-BP394170YAL
CSB-MP394170YAL
CSB-EP394170YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP394171YAL
CSB-EP394171YAL
CSB-BP394171YAL
CSB-MP394171YAL
CSB-EP394171YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Acetate kinase (ackA) CSB-YP394172YAL
CSB-EP394172YAL
CSB-BP394172YAL
CSB-MP394172YAL
CSB-EP394172YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Putative sialic acid transporter (nanT), partial CSB-YP394173YAL
CSB-EP394173YAL
CSB-BP394173YAL
CSB-MP394173YAL
CSB-EP394173YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Acetyltransferase YPDSF_2136 (YPDSF_2136) CSB-YP394174YAL
CSB-EP394174YAL
CSB-BP394174YAL
CSB-MP394174YAL
CSB-EP394174YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Uracil phosphoribosyltransferase (upp) CSB-YP394175YAL
CSB-EP394175YAL
CSB-BP394175YAL
CSB-MP394175YAL
CSB-EP394175YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP394176YAL
CSB-EP394176YAL
CSB-BP394176YAL
CSB-MP394176YAL
CSB-EP394176YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis HTH-type transcriptional regulator iscR (iscR) CSB-YP394177YAL
CSB-EP394177YAL
CSB-BP394177YAL
CSB-MP394177YAL
CSB-EP394177YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP394178YAL
CSB-EP394178YAL
CSB-BP394178YAL
CSB-MP394178YAL
CSB-EP394178YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cytidylate kinase (cmk) CSB-YP394179YAL
CSB-EP394179YAL
CSB-BP394179YAL
CSB-MP394179YAL
CSB-EP394179YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP394180YAL
CSB-EP394180YAL
CSB-BP394180YAL
CSB-MP394180YAL
CSB-EP394180YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Mannonate dehydratase (uxuA) CSB-YP394181YAL
CSB-EP394181YAL
CSB-BP394181YAL
CSB-MP394181YAL
CSB-EP394181YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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