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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas stutzeri Elongation factor Tu 1 (tuf1) CSB-YP395564PZP
CSB-EP395564PZP
CSB-BP395564PZP
CSB-MP395564PZP
CSB-EP395564PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 50S ribosomal protein L23 (rplW) CSB-YP395565PZP
CSB-EP395565PZP
CSB-BP395565PZP
CSB-MP395565PZP
CSB-EP395565PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 30S ribosomal protein S3 (rpsC) CSB-YP395566PZP
CSB-EP395566PZP
CSB-BP395566PZP
CSB-MP395566PZP
CSB-EP395566PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 50S ribosomal protein L17 (rplQ) CSB-YP395567PZP
CSB-EP395567PZP
CSB-BP395567PZP
CSB-MP395567PZP
CSB-EP395567PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Peptide chain release factor 3 (prfC), partial CSB-YP395568PZP
CSB-EP395568PZP
CSB-BP395568PZP
CSB-MP395568PZP
CSB-EP395568PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP395569PZP
CSB-EP395569PZP
CSB-BP395569PZP
CSB-MP395569PZP
CSB-EP395569PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP395570PZP
CSB-EP395570PZP
CSB-BP395570PZP
CSB-MP395570PZP
CSB-EP395570PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Electron transport complex protein RnfB (rnfB) CSB-YP395571PZP
CSB-EP395571PZP
CSB-BP395571PZP
CSB-MP395571PZP
CSB-EP395571PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Cobalamin synthase (cobS), partial CSB-YP395572PZP1
CSB-EP395572PZP1
CSB-BP395572PZP1
CSB-MP395572PZP1
CSB-EP395572PZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Probable septum site-determining protein MinC (minC) CSB-YP395573PZP
CSB-EP395573PZP
CSB-BP395573PZP
CSB-MP395573PZP
CSB-EP395573PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Uridylate kinase (pyrH) CSB-YP395574PZP
CSB-EP395574PZP
CSB-BP395574PZP
CSB-MP395574PZP
CSB-EP395574PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP395575PZP
CSB-EP395575PZP
CSB-BP395575PZP
CSB-MP395575PZP
CSB-EP395575PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP395576PZP
CSB-EP395576PZP
CSB-BP395576PZP
CSB-MP395576PZP
CSB-EP395576PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Cell division protein ZipA homolog (zipA), partial CSB-YP395577PZP
CSB-EP395577PZP
CSB-BP395577PZP
CSB-MP395577PZP
CSB-EP395577PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Coenzyme PQQ synthesis protein B (pqqB) CSB-YP395578PZP
CSB-EP395578PZP
CSB-BP395578PZP
CSB-MP395578PZP
CSB-EP395578PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri High frequency lysogenization protein HflD homolog (hflD) CSB-YP395579PZP
CSB-EP395579PZP
CSB-BP395579PZP
CSB-MP395579PZP
CSB-EP395579PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP395580PZP
CSB-EP395580PZP
CSB-BP395580PZP
CSB-MP395580PZP
CSB-EP395580PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Protease HtpX (htpX), partial CSB-YP395581PZP1
CSB-EP395581PZP1
CSB-BP395581PZP1
CSB-MP395581PZP1
CSB-EP395581PZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP395582PZP
CSB-EP395582PZP
CSB-BP395582PZP
CSB-MP395582PZP
CSB-EP395582PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP395583PZP
CSB-EP395583PZP
CSB-BP395583PZP
CSB-MP395583PZP
CSB-EP395583PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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