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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP395764RLG
CSB-EP395764RLG
CSB-BP395764RLG
CSB-MP395764RLG
CSB-EP395764RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP395765RLG
CSB-EP395765RLG
CSB-BP395765RLG
CSB-MP395765RLG
CSB-EP395765RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S5 (rpsE) CSB-YP395766RLG
CSB-EP395766RLG
CSB-BP395766RLG
CSB-MP395766RLG
CSB-EP395766RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L5 (rplE) CSB-YP395767RLG
CSB-EP395767RLG
CSB-BP395767RLG
CSB-MP395767RLG
CSB-EP395767RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S3 (rpsC) CSB-YP395768RLG
CSB-EP395768RLG
CSB-BP395768RLG
CSB-MP395768RLG
CSB-EP395768RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L4 (rplD) CSB-YP395769RLG
CSB-EP395769RLG
CSB-BP395769RLG
CSB-MP395769RLG
CSB-EP395769RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP395770RLG
CSB-EP395770RLG
CSB-BP395770RLG
CSB-MP395770RLG
CSB-EP395770RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP395771RLG
CSB-EP395771RLG
CSB-BP395771RLG
CSB-MP395771RLG
CSB-EP395771RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Protein translocase subunit SecA (secA), partial CSB-YP395772RLG
CSB-EP395772RLG
CSB-BP395772RLG
CSB-MP395772RLG
CSB-EP395772RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP395773RLG
CSB-EP395773RLG
CSB-BP395773RLG
CSB-MP395773RLG
CSB-EP395773RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Dihydrodipicolinate reductase (dapB) CSB-YP395774RLG
CSB-EP395774RLG
CSB-BP395774RLG
CSB-MP395774RLG
CSB-EP395774RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Glutamate--tRNA ligase 2 (gltX2) CSB-YP395775RLG
CSB-EP395775RLG
CSB-BP395775RLG
CSB-MP395775RLG
CSB-EP395775RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Leucine--tRNA ligase (leuS), partial CSB-YP395776RLG
CSB-EP395776RLG
CSB-BP395776RLG
CSB-MP395776RLG
CSB-EP395776RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Mycothiol acetyltransferase (mshD) CSB-YP395777STD
CSB-EP395777STD
CSB-BP395777STD
CSB-MP395777STD
CSB-EP395777STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP395778STD
CSB-EP395778STD
CSB-BP395778STD
CSB-MP395778STD
CSB-EP395778STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Na (+)/H (+) antiporter nhaA 2, partial CSB-YP395779STD
CSB-EP395779STD
CSB-BP395779STD
CSB-MP395779STD
CSB-EP395779STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Phosphopantetheine adenylyltransferase (coaD) CSB-YP395780STD
CSB-EP395780STD
CSB-BP395780STD
CSB-MP395780STD
CSB-EP395780STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP395781STD
CSB-EP395781STD
CSB-BP395781STD
CSB-MP395781STD
CSB-EP395781STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP395782STD
CSB-EP395782STD
CSB-BP395782STD
CSB-MP395782STD
CSB-EP395782STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 3-dehydroquinate dehydratase (aroQ) CSB-YP395783STD
CSB-EP395783STD
CSB-BP395783STD
CSB-MP395783STD
CSB-EP395783STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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