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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant European bat lyssavirus 1 Phosphoprotein (P) CSB-YP394223EJH
CSB-EP394223EJH
CSB-BP394223EJH
CSB-MP394223EJH
CSB-EP394223EJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European bat lyssavirus 2 Glycoprotein G (G), partial CSB-YP394224EJI1
CSB-EP394224EJI1
CSB-BP394224EJI1
CSB-MP394224EJI1
CSB-EP394224EJI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii S-layer biosynthesis glycosyltransferase AglD (aglD) CSB-YP394225HTT
CSB-EP394225HTT
CSB-BP394225HTT
CSB-MP394225HTT
CSB-EP394225HTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Glutamate--cysteine ligase (gshA), partial CSB-YP394226PZP
CSB-EP394226PZP
CSB-BP394226PZP
CSB-MP394226PZP
CSB-EP394226PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP394227PZP
CSB-EP394227PZP
CSB-BP394227PZP
CSB-MP394227PZP
CSB-EP394227PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribose-5-phosphate isomerase A (rpiA) CSB-YP394228PZP
CSB-EP394228PZP
CSB-BP394228PZP
CSB-MP394228PZP
CSB-EP394228PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Orotate phosphoribosyltransferase (pyrE) CSB-YP394229PZP
CSB-EP394229PZP
CSB-BP394229PZP
CSB-MP394229PZP
CSB-EP394229PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0178 protein PST_0536 (PST_0536) CSB-YP394230PZP
CSB-EP394230PZP
CSB-BP394230PZP
CSB-MP394230PZP
CSB-EP394230PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0229 protein PST_0721 (PST_0721) CSB-YP394231PZP
CSB-EP394231PZP
CSB-BP394231PZP
CSB-MP394231PZP
CSB-EP394231PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Thiosulfate sulfurtransferase glpE (glpE) CSB-YP394232PZP
CSB-EP394232PZP
CSB-BP394232PZP
CSB-MP394232PZP
CSB-EP394232PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 50S ribosomal protein L10 (rplJ) CSB-YP394233PZP
CSB-EP394233PZP
CSB-BP394233PZP
CSB-MP394233PZP
CSB-EP394233PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 30S ribosomal protein S12 (rpsL) CSB-YP394234PZP
CSB-EP394234PZP
CSB-BP394234PZP
CSB-MP394234PZP
CSB-EP394234PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 50S ribosomal protein L2 (rplB) CSB-YP394235PZP
CSB-EP394235PZP
CSB-BP394235PZP
CSB-MP394235PZP
CSB-EP394235PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 50S ribosomal protein L24 (rplX) CSB-YP394236PZP
CSB-EP394236PZP
CSB-BP394236PZP
CSB-MP394236PZP
CSB-EP394236PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri N utilization substance protein B homolog (nusB) CSB-YP394237PZP
CSB-EP394237PZP
CSB-BP394237PZP
CSB-MP394237PZP
CSB-EP394237PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0114 protein PST_0950 (PST_0950), partial CSB-YP394238PZP1
CSB-EP394238PZP1
CSB-BP394238PZP1
CSB-MP394238PZP1
CSB-EP394238PZP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 30S ribosomal protein S9 (rpsI) CSB-YP394239PZP
CSB-EP394239PZP
CSB-BP394239PZP
CSB-MP394239PZP
CSB-EP394239PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0246 protein PST_1170 (PST_1170) CSB-YP394240PZP
CSB-EP394240PZP
CSB-BP394240PZP
CSB-MP394240PZP
CSB-EP394240PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri tRNA (cmo5U34)-methyltransferase CSB-YP394241PZP
CSB-EP394241PZP
CSB-BP394241PZP
CSB-MP394241PZP
CSB-EP394241PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP394242PZP
CSB-EP394242PZP
CSB-BP394242PZP
CSB-MP394242PZP
CSB-EP394242PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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