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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis 8-amino-7-oxononanoate synthase (bioF) CSB-YP394824YAL
CSB-EP394824YAL
CSB-BP394824YAL
CSB-MP394824YAL
CSB-EP394824YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis LPS-assembly lipoprotein lptE (lptE) CSB-YP394825YAL
CSB-EP394825YAL
CSB-BP394825YAL
CSB-MP394825YAL
CSB-EP394825YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP394826YAL
CSB-EP394826YAL
CSB-BP394826YAL
CSB-MP394826YAL
CSB-EP394826YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Glutamate 5-kinase (proB) CSB-YP394827YAL
CSB-EP394827YAL
CSB-BP394827YAL
CSB-MP394827YAL
CSB-EP394827YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Na (+)-translocating NADH-quinone reductase subunit F, partial CSB-YP394828YAL
CSB-EP394828YAL
CSB-BP394828YAL
CSB-MP394828YAL
CSB-EP394828YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0289 protein YPDSF_2924 (YPDSF_2924) CSB-YP394829YAL
CSB-EP394829YAL
CSB-BP394829YAL
CSB-MP394829YAL
CSB-EP394829YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP394830YAL
CSB-EP394830YAL
CSB-BP394830YAL
CSB-MP394830YAL
CSB-EP394830YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 5'/3'-nucleotidase SurE (surE) CSB-YP394831YAL
CSB-EP394831YAL
CSB-BP394831YAL
CSB-MP394831YAL
CSB-EP394831YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Alanine--tRNA ligase (alaS), partial CSB-YP394832YAL
CSB-EP394832YAL
CSB-BP394832YAL
CSB-MP394832YAL
CSB-EP394832YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Secretion monitor (secM) CSB-YP394833YAL
CSB-EP394833YAL
CSB-BP394833YAL
CSB-MP394833YAL
CSB-EP394833YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP394834YAL
CSB-EP394834YAL
CSB-BP394834YAL
CSB-MP394834YAL
CSB-EP394834YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 2-isopropylmalate synthase (leuA), partial CSB-YP394835YAL
CSB-EP394835YAL
CSB-BP394835YAL
CSB-MP394835YAL
CSB-EP394835YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP394836YAL
CSB-EP394836YAL
CSB-BP394836YAL
CSB-MP394836YAL
CSB-EP394836YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 30S ribosomal protein S20 (rpsT) CSB-YP394837YAL
CSB-EP394837YAL
CSB-BP394837YAL
CSB-MP394837YAL
CSB-EP394837YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP394838YAL
CSB-EP394838YAL
CSB-BP394838YAL
CSB-MP394838YAL
CSB-EP394838YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Autoinducer 2 import system permease protein lsrC (lsrC), partial CSB-YP394839YAL
CSB-EP394839YAL
CSB-BP394839YAL
CSB-MP394839YAL
CSB-EP394839YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0213 protein YPDSF_3285 (YPDSF_3285) CSB-YP394840YAL
CSB-EP394840YAL
CSB-BP394840YAL
CSB-MP394840YAL
CSB-EP394840YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Universal stress protein B (uspB), partial CSB-YP394841YAL1
CSB-EP394841YAL1
CSB-BP394841YAL1
CSB-MP394841YAL1
CSB-EP394841YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP394842YAL
CSB-EP394842YAL
CSB-BP394842YAL
CSB-MP394842YAL
CSB-EP394842YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA (uracil (54)-C (5))-methyltransferase CSB-YP394843YAL
CSB-EP394843YAL
CSB-BP394843YAL
CSB-MP394843YAL
CSB-EP394843YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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