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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L16 (rplP) CSB-YP396104RLG
CSB-EP396104RLG
CSB-BP396104RLG
CSB-MP396104RLG
CSB-EP396104RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L1 (rplA) CSB-YP396105RLG
CSB-EP396105RLG
CSB-BP396105RLG
CSB-MP396105RLG
CSB-EP396105RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP396106RLG
CSB-EP396106RLG
CSB-BP396106RLG
CSB-MP396106RLG
CSB-EP396106RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides DNA mismatch repair protein MutS (mutS), partial CSB-YP396107RLG
CSB-EP396107RLG
CSB-BP396107RLG
CSB-MP396107RLG
CSB-EP396107RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Shikimate dehydrogenase (aroE) CSB-YP396108RLG
CSB-EP396108RLG
CSB-BP396108RLG
CSB-MP396108RLG
CSB-EP396108RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Chaperone protein DnaK (dnaK), partial CSB-YP396109RLG
CSB-EP396109RLG
CSB-BP396109RLG
CSB-MP396109RLG
CSB-EP396109RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP396110RLG
CSB-EP396110RLG
CSB-BP396110RLG
CSB-MP396110RLG
CSB-EP396110RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP396111RLG
CSB-EP396111RLG
CSB-BP396111RLG
CSB-MP396111RLG
CSB-EP396111RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP-dependent protease subunit HslV (hslV) CSB-YP396112RLG
CSB-EP396112RLG
CSB-BP396112RLG
CSB-MP396112RLG
CSB-EP396112RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Heme A synthase (ctaA), partial CSB-YP396113RLG
CSB-EP396113RLG
CSB-BP396113RLG
CSB-MP396113RLG
CSB-EP396113RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica UPF0678 fatty acid-binding protein-like protein Strop_0310 (Strop_0310) CSB-YP396114STD
CSB-EP396114STD
CSB-BP396114STD
CSB-MP396114STD
CSB-EP396114STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica NADH-quinone oxidoreductase subunit B 1 (nuoB1) CSB-YP396115STD
CSB-EP396115STD
CSB-BP396115STD
CSB-MP396115STD
CSB-EP396115STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribonuclease PH (rph) CSB-YP396116STD
CSB-EP396116STD
CSB-BP396116STD
CSB-MP396116STD
CSB-EP396116STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica DNA ligase (ligA), partial CSB-YP396117STD
CSB-EP396117STD
CSB-BP396117STD
CSB-MP396117STD
CSB-EP396117STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribonuclease HII (rnhB) CSB-YP396118STD
CSB-EP396118STD
CSB-BP396118STD
CSB-MP396118STD
CSB-EP396118STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribosome maturation factor RimP (rimP) CSB-YP396119STD
CSB-EP396119STD
CSB-BP396119STD
CSB-MP396119STD
CSB-EP396119STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Na (+)/H (+) antiporter nhaA 3, partial CSB-YP396120STD
CSB-EP396120STD
CSB-BP396120STD
CSB-MP396120STD
CSB-EP396120STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Aspartate carbamoyltransferase (pyrB) CSB-YP396121STD
CSB-EP396121STD
CSB-BP396121STD
CSB-MP396121STD
CSB-EP396121STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP396122STD
CSB-EP396122STD
CSB-BP396122STD
CSB-MP396122STD
CSB-EP396122STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP396123STD
CSB-EP396123STD
CSB-BP396123STD
CSB-MP396123STD
CSB-EP396123STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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