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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP395504YAL
CSB-EP395504YAL
CSB-BP395504YAL
CSB-MP395504YAL
CSB-EP395504YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Urease subunit alpha (ureC), partial CSB-YP395505YAL
CSB-EP395505YAL
CSB-BP395505YAL
CSB-MP395505YAL
CSB-EP395505YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribonuclease H (rnhA) CSB-YP395506YAL
CSB-EP395506YAL
CSB-BP395506YAL
CSB-MP395506YAL
CSB-EP395506YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribonuclease HII (rnhB) CSB-YP395507YAL
CSB-EP395507YAL
CSB-BP395507YAL
CSB-MP395507YAL
CSB-EP395507YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 30S ribosomal protein S2 (rpsB) CSB-YP395508YAL
CSB-EP395508YAL
CSB-BP395508YAL
CSB-MP395508YAL
CSB-EP395508YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0325 protein YPDSF_1673 (YPDSF_1673) CSB-YP395509YAL
CSB-EP395509YAL
CSB-BP395509YAL
CSB-MP395509YAL
CSB-EP395509YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Bifunctional protein aas (aas), partial CSB-YP395510YAL1
CSB-EP395510YAL1
CSB-BP395510YAL1
CSB-MP395510YAL1
CSB-EP395510YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP395511YAL
CSB-EP395511YAL
CSB-BP395511YAL
CSB-MP395511YAL
CSB-EP395511YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0207 protein YPDSF_1968 (YPDSF_1968) CSB-YP395512YAL
CSB-EP395512YAL
CSB-BP395512YAL
CSB-MP395512YAL
CSB-EP395512YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP395513YAL
CSB-EP395513YAL
CSB-BP395513YAL
CSB-MP395513YAL
CSB-EP395513YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP395514YAL
CSB-EP395514YAL
CSB-BP395514YAL
CSB-MP395514YAL
CSB-EP395514YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP395515YAL
CSB-EP395515YAL
CSB-BP395515YAL
CSB-MP395515YAL
CSB-EP395515YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0370 protein YPDSF_2160 (YPDSF_2160), partial CSB-YP395516YAL
CSB-EP395516YAL
CSB-BP395516YAL
CSB-MP395516YAL
CSB-EP395516YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis GTPase Der (der) CSB-YP395517YAL
CSB-EP395517YAL
CSB-BP395517YAL
CSB-MP395517YAL
CSB-EP395517YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP395518YAL
CSB-EP395518YAL
CSB-BP395518YAL
CSB-MP395518YAL
CSB-EP395518YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Serine--tRNA ligase (serS) CSB-YP395519YAL
CSB-EP395519YAL
CSB-BP395519YAL
CSB-MP395519YAL
CSB-EP395519YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Putative transport protein YPDSF_2370 (YPDSF_2370), partial CSB-YP395520YAL
CSB-EP395520YAL
CSB-BP395520YAL
CSB-MP395520YAL
CSB-EP395520YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP395521YAL
CSB-EP395521YAL
CSB-BP395521YAL
CSB-MP395521YAL
CSB-EP395521YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Protein RnfH (rnfH) CSB-YP395522YAL
CSB-EP395522YAL
CSB-BP395522YAL
CSB-MP395522YAL
CSB-EP395522YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP395523YAL
CSB-EP395523YAL
CSB-BP395523YAL
CSB-MP395523YAL
CSB-EP395523YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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