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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas stutzeri UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP395904PZP
CSB-EP395904PZP
CSB-BP395904PZP
CSB-MP395904PZP
CSB-EP395904PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosome maturation factor RimM (rimM) CSB-YP395905PZP
CSB-EP395905PZP
CSB-BP395905PZP
CSB-MP395905PZP
CSB-EP395905PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri DNA repair protein recO (recO) CSB-YP395906PZP
CSB-EP395906PZP
CSB-BP395906PZP
CSB-MP395906PZP
CSB-EP395906PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP395907PZP
CSB-EP395907PZP
CSB-BP395907PZP
CSB-MP395907PZP
CSB-EP395907PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Flagellar L-ring protein (flgH) CSB-YP395908PZP
CSB-EP395908PZP
CSB-BP395908PZP
CSB-MP395908PZP
CSB-EP395908PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP395909PZP
CSB-EP395909PZP
CSB-BP395909PZP
CSB-MP395909PZP
CSB-EP395909PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP395910PZP
CSB-EP395910PZP
CSB-BP395910PZP
CSB-MP395910PZP
CSB-EP395910PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Catalase-peroxidase (katG), partial CSB-YP395911PZP
CSB-EP395911PZP
CSB-BP395911PZP
CSB-MP395911PZP
CSB-EP395911PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP395912PZP
CSB-EP395912PZP
CSB-BP395912PZP
CSB-MP395912PZP
CSB-EP395912PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri DNA ligase (ligA), partial CSB-YP395913PZP
CSB-EP395913PZP
CSB-BP395913PZP
CSB-MP395913PZP
CSB-EP395913PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Putative regulator of ribonuclease activity (PST_2040) CSB-YP395914PZP
CSB-EP395914PZP
CSB-BP395914PZP
CSB-MP395914PZP
CSB-EP395914PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Probable RNA methyltransferase PST_2231 (PST_2231) CSB-YP395915PZP
CSB-EP395915PZP
CSB-BP395915PZP
CSB-MP395915PZP
CSB-EP395915PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP395916PZP
CSB-EP395916PZP
CSB-BP395916PZP
CSB-MP395916PZP
CSB-EP395916PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP395917PZP
CSB-EP395917PZP
CSB-BP395917PZP
CSB-MP395917PZP
CSB-EP395917PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP395918PZP
CSB-EP395918PZP
CSB-BP395918PZP
CSB-MP395918PZP
CSB-EP395918PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri 50S ribosomal protein L32 (rpmF) CSB-YP395919PZP
CSB-EP395919PZP
CSB-BP395919PZP
CSB-MP395919PZP
CSB-EP395919PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri UPF0317 protein PST_2764 (PST_2764) CSB-YP395920PZP
CSB-EP395920PZP
CSB-BP395920PZP
CSB-MP395920PZP
CSB-EP395920PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Protein slyX homolog (slyX) CSB-YP395921PZP
CSB-EP395921PZP
CSB-BP395921PZP
CSB-MP395921PZP
CSB-EP395921PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP395922PZP
CSB-EP395922PZP
CSB-BP395922PZP
CSB-MP395922PZP
CSB-EP395922PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP395923PZP
CSB-EP395923PZP
CSB-BP395923PZP
CSB-MP395923PZP
CSB-EP395923PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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