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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter sphaeroides Transcription elongation factor GreA (greA) CSB-YP395084RLG
CSB-EP395084RLG
CSB-BP395084RLG
CSB-MP395084RLG
CSB-EP395084RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L20 (rplT) CSB-YP395085RLG
CSB-EP395085RLG
CSB-BP395085RLG
CSB-MP395085RLG
CSB-EP395085RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S14 (rpsN) CSB-YP395086RLG
CSB-EP395086RLG
CSB-BP395086RLG
CSB-MP395086RLG
CSB-EP395086RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S19 (rpsS) CSB-YP395087RLG
CSB-EP395087RLG
CSB-BP395087RLG
CSB-MP395087RLG
CSB-EP395087RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 50S ribosomal protein L10 (rplJ) CSB-YP395088RLG
CSB-EP395088RLG
CSB-BP395088RLG
CSB-MP395088RLG
CSB-EP395088RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP395089RLG
CSB-EP395089RLG
CSB-BP395089RLG
CSB-MP395089RLG
CSB-EP395089RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Xylose isomerase (xylA) CSB-YP395090RLG
CSB-EP395090RLG
CSB-BP395090RLG
CSB-MP395090RLG
CSB-EP395090RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP395091RLG
CSB-EP395091RLG
CSB-BP395091RLG
CSB-MP395091RLG
CSB-EP395091RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP395092RLG
CSB-EP395092RLG
CSB-BP395092RLG
CSB-MP395092RLG
CSB-EP395092RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 30S ribosomal protein S15 (rpsO) CSB-YP395093RLG
CSB-EP395093RLG
CSB-BP395093RLG
CSB-MP395093RLG
CSB-EP395093RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP395094RLG
CSB-EP395094RLG
CSB-BP395094RLG
CSB-MP395094RLG
CSB-EP395094RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP395095RLG
CSB-EP395095RLG
CSB-BP395095RLG
CSB-MP395095RLG
CSB-EP395095RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Fructose-1,6-bisphosphatase class 1 2 (fbp2) CSB-YP395096RLG
CSB-EP395096RLG
CSB-BP395096RLG
CSB-MP395096RLG
CSB-EP395096RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Chaperone protein DnaK (dnaK), partial CSB-YP395097STD
CSB-EP395097STD
CSB-BP395097STD
CSB-MP395097STD
CSB-EP395097STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Glutamyl-tRNA reductase (hemA) CSB-YP395098STD
CSB-EP395098STD
CSB-BP395098STD
CSB-MP395098STD
CSB-EP395098STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Maf-like protein Strop_0851 (Strop_0851) CSB-YP395099STD
CSB-EP395099STD
CSB-BP395099STD
CSB-MP395099STD
CSB-EP395099STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP395100STD
CSB-EP395100STD
CSB-BP395100STD
CSB-MP395100STD
CSB-EP395100STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP395101STD
CSB-EP395101STD
CSB-BP395101STD
CSB-MP395101STD
CSB-EP395101STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP395102STD
CSB-EP395102STD
CSB-BP395102STD
CSB-MP395102STD
CSB-EP395102STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica tRNA (guanine-N (1)-)-methyltransferase CSB-YP395103STD
CSB-EP395103STD
CSB-BP395103STD
CSB-MP395103STD
CSB-EP395103STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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