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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Caldicellulosiruptor saccharolyticus Ribonuclease HII (rnhB) CSB-YP395144CVX
CSB-EP395144CVX
CSB-BP395144CVX
CSB-MP395144CVX
CSB-EP395144CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP395145CVX
CSB-EP395145CVX
CSB-BP395145CVX
CSB-MP395145CVX
CSB-EP395145CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Adenylate kinase (adk) CSB-YP395146CVX
CSB-EP395146CVX
CSB-BP395146CVX
CSB-MP395146CVX
CSB-EP395146CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP395147CVX
CSB-EP395147CVX
CSB-BP395147CVX
CSB-MP395147CVX
CSB-EP395147CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 50S ribosomal protein L23 (rplW) CSB-YP395148CVX
CSB-EP395148CVX
CSB-BP395148CVX
CSB-MP395148CVX
CSB-EP395148CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP395149CVX
CSB-EP395149CVX
CSB-BP395149CVX
CSB-MP395149CVX
CSB-EP395149CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP395150CVX
CSB-EP395150CVX
CSB-BP395150CVX
CSB-MP395150CVX
CSB-EP395150CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Adenylosuccinate synthetase (purA) CSB-YP395151CVX
CSB-EP395151CVX
CSB-BP395151CVX
CSB-MP395151CVX
CSB-EP395151CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP395152CVX
CSB-EP395152CVX
CSB-BP395152CVX
CSB-MP395152CVX
CSB-EP395152CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP395153PZM
CSB-EP395153PZM
CSB-BP395153PZM
CSB-MP395153PZM
CSB-EP395153PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0299 membrane protein YPDSF_1460 (YPDSF_1460), partial CSB-YP395154YAL1
CSB-EP395154YAL1
CSB-BP395154YAL1
CSB-MP395154YAL1
CSB-EP395154YAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP395155YAL
CSB-EP395155YAL
CSB-BP395155YAL
CSB-MP395155YAL
CSB-EP395155YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP395156YAL
CSB-EP395156YAL
CSB-BP395156YAL
CSB-MP395156YAL
CSB-EP395156YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0249 protein YPDSF_1592 (YPDSF_1592) CSB-YP395157YAL
CSB-EP395157YAL
CSB-BP395157YAL
CSB-MP395157YAL
CSB-EP395157YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP395158YAL
CSB-EP395158YAL
CSB-BP395158YAL
CSB-MP395158YAL
CSB-EP395158YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis RNA pyrophosphohydrolase (rppH) CSB-YP395159YAL
CSB-EP395159YAL
CSB-BP395159YAL
CSB-MP395159YAL
CSB-EP395159YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP395160YAL
CSB-EP395160YAL
CSB-BP395160YAL
CSB-MP395160YAL
CSB-EP395160YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Trans-aconitate 2-methyltransferase (tam) CSB-YP395161YAL
CSB-EP395161YAL
CSB-BP395161YAL
CSB-MP395161YAL
CSB-EP395161YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP395162YAL
CSB-EP395162YAL
CSB-BP395162YAL
CSB-MP395162YAL
CSB-EP395162YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis UPF0304 protein YPDSF_1971 (YPDSF_1971) CSB-YP395163YAL
CSB-EP395163YAL
CSB-BP395163YAL
CSB-MP395163YAL
CSB-EP395163YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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