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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. UPF0266 membrane protein Ent638_2389 (Ent638_2389), partial CSB-YP395004EIZ1
CSB-EP395004EIZ1
CSB-BP395004EIZ1
CSB-MP395004EIZ1
CSB-EP395004EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP395005EIZ
CSB-EP395005EIZ
CSB-BP395005EIZ
CSB-MP395005EIZ
CSB-EP395005EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. tRNA (cmo5U34)-methyltransferase CSB-YP395006EIZ
CSB-EP395006EIZ
CSB-BP395006EIZ
CSB-MP395006EIZ
CSB-EP395006EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Flagellar protein FliT (fliT) CSB-YP395007EIZ
CSB-EP395007EIZ
CSB-BP395007EIZ
CSB-MP395007EIZ
CSB-EP395007EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP395008EIZ
CSB-EP395008EIZ
CSB-BP395008EIZ
CSB-MP395008EIZ
CSB-EP395008EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Nucleoid-associated protein Ent638_2782 (Ent638_2782) CSB-YP395009EIZ
CSB-EP395009EIZ
CSB-BP395009EIZ
CSB-MP395009EIZ
CSB-EP395009EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. o-succinylbenzoate synthase (menC) CSB-YP395010EIZ
CSB-EP395010EIZ
CSB-BP395010EIZ
CSB-MP395010EIZ
CSB-EP395010EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Ribonuclease BN (rbn) CSB-YP395011EIZ
CSB-EP395011EIZ
CSB-BP395011EIZ
CSB-MP395011EIZ
CSB-EP395011EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Glutamate--tRNA ligase (gltX) CSB-YP395012EIZ
CSB-EP395012EIZ
CSB-BP395012EIZ
CSB-MP395012EIZ
CSB-EP395012EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0370 protein Ent638_2968 (Ent638_2968), partial CSB-YP395013EIZ
CSB-EP395013EIZ
CSB-BP395013EIZ
CSB-MP395013EIZ
CSB-EP395013EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP395014EIZ
CSB-EP395014EIZ
CSB-BP395014EIZ
CSB-MP395014EIZ
CSB-EP395014EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Co-chaperone protein hscB (hscB) CSB-YP395015EIZ
CSB-EP395015EIZ
CSB-BP395015EIZ
CSB-MP395015EIZ
CSB-EP395015EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP395016EIZ
CSB-EP395016EIZ
CSB-BP395016EIZ
CSB-MP395016EIZ
CSB-EP395016EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Elongation factor 4 (lepA), partial CSB-YP395017EIZ
CSB-EP395017EIZ
CSB-BP395017EIZ
CSB-MP395017EIZ
CSB-EP395017EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. SsrA-binding protein (smpB) CSB-YP395018EIZ
CSB-EP395018EIZ
CSB-BP395018EIZ
CSB-MP395018EIZ
CSB-EP395018EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein RecA (recA) CSB-YP395019EIZ
CSB-EP395019EIZ
CSB-BP395019EIZ
CSB-MP395019EIZ
CSB-EP395019EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. DNA mismatch repair protein MutS (mutS), partial CSB-YP395020EIZ
CSB-EP395020EIZ
CSB-BP395020EIZ
CSB-MP395020EIZ
CSB-EP395020EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP395021EIZ
CSB-EP395021EIZ
CSB-BP395021EIZ
CSB-MP395021EIZ
CSB-EP395021EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. tRNA-modifying protein ygfZ (Ent638_3316) CSB-YP395022EIZ
CSB-EP395022EIZ
CSB-BP395022EIZ
CSB-MP395022EIZ
CSB-EP395022EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Arginine exporter protein ArgO (argO), partial CSB-YP395023EIZ1
CSB-EP395023EIZ1
CSB-BP395023EIZ1
CSB-MP395023EIZ1
CSB-EP395023EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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