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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter sp. UPF0114 protein Ent638_3411 (Ent638_3411), partial CSB-YP395024EIZ1
CSB-EP395024EIZ1
CSB-BP395024EIZ1
CSB-MP395024EIZ1
CSB-EP395024EIZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Putrescine aminotransferase (patA) CSB-YP395025EIZ
CSB-EP395025EIZ
CSB-BP395025EIZ
CSB-MP395025EIZ
CSB-EP395025EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP395026EIZ
CSB-EP395026EIZ
CSB-BP395026EIZ
CSB-MP395026EIZ
CSB-EP395026EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. GTPase obg (obg) CSB-YP395027EIZ
CSB-EP395027EIZ
CSB-BP395027EIZ
CSB-MP395027EIZ
CSB-EP395027EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Probable cytosol aminopeptidase (pepA), partial CSB-YP395028EIZ
CSB-EP395028EIZ
CSB-BP395028EIZ
CSB-MP395028EIZ
CSB-EP395028EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein smg (smg) CSB-YP395029EIZ
CSB-EP395029EIZ
CSB-BP395029EIZ
CSB-MP395029EIZ
CSB-EP395029EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 30S ribosomal protein S4 (rpsD) CSB-YP395030EIZ
CSB-EP395030EIZ
CSB-BP395030EIZ
CSB-MP395030EIZ
CSB-EP395030EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L36 1 (rpmJ1) CSB-YP395031EIZ
CSB-EP395031EIZ
CSB-BP395031EIZ
CSB-MP395031EIZ
CSB-EP395031EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 30S ribosomal protein S14 (rpsN) CSB-YP395032EIZ
CSB-EP395032EIZ
CSB-BP395032EIZ
CSB-MP395032EIZ
CSB-EP395032EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 30S ribosomal protein S17 (rpsQ) CSB-YP395033EIZ
CSB-EP395033EIZ
CSB-BP395033EIZ
CSB-MP395033EIZ
CSB-EP395033EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L22 (rplV) CSB-YP395034EIZ
CSB-EP395034EIZ
CSB-BP395034EIZ
CSB-MP395034EIZ
CSB-EP395034EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L3 (rplC) CSB-YP395035EIZ
CSB-EP395035EIZ
CSB-BP395035EIZ
CSB-MP395035EIZ
CSB-EP395035EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Protein slyX (slyX) CSB-YP395036EIZ
CSB-EP395036EIZ
CSB-BP395036EIZ
CSB-MP395036EIZ
CSB-EP395036EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. UPF0270 protein Ent638_3781 (Ent638_3781) CSB-YP395037EIZ
CSB-EP395037EIZ
CSB-BP395037EIZ
CSB-MP395037EIZ
CSB-EP395037EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 33 kDa chaperonin (hslO) CSB-YP395038EIZ
CSB-EP395038EIZ
CSB-BP395038EIZ
CSB-MP395038EIZ
CSB-EP395038EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Fe/S biogenesis protein NfuA (nfuA) CSB-YP395039EIZ
CSB-EP395039EIZ
CSB-BP395039EIZ
CSB-MP395039EIZ
CSB-EP395039EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Rhomboid protease glpG (glpG), partial CSB-YP395040EIZ1
CSB-EP395040EIZ1
CSB-BP395040EIZ1
CSB-MP395040EIZ1
CSB-EP395040EIZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP395041EIZ
CSB-EP395041EIZ
CSB-BP395041EIZ
CSB-MP395041EIZ
CSB-EP395041EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Diaminopimelate epimerase (dapF) CSB-YP395042EIZ
CSB-EP395042EIZ
CSB-BP395042EIZ
CSB-MP395042EIZ
CSB-EP395042EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. tRNA (uracil (54)-C (5))-methyltransferase CSB-YP395043EIZ
CSB-EP395043EIZ
CSB-BP395043EIZ
CSB-MP395043EIZ
CSB-EP395043EIZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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