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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella putrefaciens Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP397185STQ
CSB-EP397185STQ
CSB-BP397185STQ
CSB-MP397185STQ
CSB-EP397185STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP397186STQ
CSB-EP397186STQ
CSB-BP397186STQ
CSB-MP397186STQ
CSB-EP397186STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribonuclease T (rnt) CSB-YP397187STQ
CSB-EP397187STQ
CSB-BP397187STQ
CSB-MP397187STQ
CSB-EP397187STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Biosynthetic arginine decarboxylase (speA), partial CSB-YP397188STQ
CSB-EP397188STQ
CSB-BP397188STQ
CSB-MP397188STQ
CSB-EP397188STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Cell division topological specificity factor (minE) CSB-YP397189STQ
CSB-EP397189STQ
CSB-BP397189STQ
CSB-MP397189STQ
CSB-EP397189STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP397190STQ
CSB-EP397190STQ
CSB-BP397190STQ
CSB-MP397190STQ
CSB-EP397190STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Electron transport complex protein RnfA (rnfA), partial CSB-YP397191STQ1
CSB-EP397191STQ1
CSB-BP397191STQ1
CSB-MP397191STQ1
CSB-EP397191STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP397192STQ
CSB-EP397192STQ
CSB-BP397192STQ
CSB-MP397192STQ
CSB-EP397192STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal protein S6 modification protein 1 (rimK1) CSB-YP397193STQ
CSB-EP397193STQ
CSB-BP397193STQ
CSB-MP397193STQ
CSB-EP397193STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Translation initiation factor IF-1 (infA) CSB-YP397194STQ
CSB-EP397194STQ
CSB-BP397194STQ
CSB-MP397194STQ
CSB-EP397194STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Putative phosphotransferase Sputcn32_2244 (Sputcn32_2244) CSB-YP397195STQ
CSB-EP397195STQ
CSB-BP397195STQ
CSB-MP397195STQ
CSB-EP397195STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Recombination protein RecR (recR) CSB-YP397196STQ
CSB-EP397196STQ
CSB-BP397196STQ
CSB-MP397196STQ
CSB-EP397196STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens DNA ligase (ligA), partial CSB-YP397197STQ
CSB-EP397197STQ
CSB-BP397197STQ
CSB-MP397197STQ
CSB-EP397197STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Chorismate synthase (aroC) CSB-YP397198STQ
CSB-EP397198STQ
CSB-BP397198STQ
CSB-MP397198STQ
CSB-EP397198STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP397199STQ
CSB-EP397199STQ
CSB-BP397199STQ
CSB-MP397199STQ
CSB-EP397199STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0301 protein Sputcn32_2681 (Sputcn32_2681) CSB-YP397200STQ
CSB-EP397200STQ
CSB-BP397200STQ
CSB-MP397200STQ
CSB-EP397200STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens tRNA (guanine-N (7)-)-methyltransferase CSB-YP397201STQ
CSB-EP397201STQ
CSB-BP397201STQ
CSB-MP397201STQ
CSB-EP397201STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phosphopentomutase (deoB) CSB-YP397202STQ
CSB-EP397202STQ
CSB-BP397202STQ
CSB-MP397202STQ
CSB-EP397202STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0250 protein Sputcn32_2874 (Sputcn32_2874) CSB-YP397203STQ
CSB-EP397203STQ
CSB-BP397203STQ
CSB-MP397203STQ
CSB-EP397203STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP397204STQ
CSB-EP397204STQ
CSB-BP397204STQ
CSB-MP397204STQ
CSB-EP397204STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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