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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella putrefaciens UPF0115 protein Sputcn32_2453 (Sputcn32_2453) CSB-YP397586STQ
CSB-EP397586STQ
CSB-BP397586STQ
CSB-MP397586STQ
CSB-EP397586STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens GTPase Der (der) CSB-YP397587STQ
CSB-EP397587STQ
CSB-BP397587STQ
CSB-MP397587STQ
CSB-EP397587STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP397588STQ
CSB-EP397588STQ
CSB-BP397588STQ
CSB-MP397588STQ
CSB-EP397588STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Probable Fe (2+)-trafficking protein CSB-YP397589STQ
CSB-EP397589STQ
CSB-BP397589STQ
CSB-MP397589STQ
CSB-EP397589STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens DNA mismatch repair protein MutS (mutS), partial CSB-YP397590STQ
CSB-EP397590STQ
CSB-BP397590STQ
CSB-MP397590STQ
CSB-EP397590STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP397591STQ
CSB-EP397591STQ
CSB-BP397591STQ
CSB-MP397591STQ
CSB-EP397591STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosome-binding factor A (rbfA) CSB-YP397592STQ
CSB-EP397592STQ
CSB-BP397592STQ
CSB-MP397592STQ
CSB-EP397592STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Phosphoglucosamine mutase (glmM) CSB-YP397593STQ
CSB-EP397593STQ
CSB-BP397593STQ
CSB-MP397593STQ
CSB-EP397593STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Probable rRNA maturation factor (Sputcn32_2857) CSB-YP397594STQ
CSB-EP397594STQ
CSB-BP397594STQ
CSB-MP397594STQ
CSB-EP397594STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Octanoyltransferase (lipB) CSB-YP397595STQ
CSB-EP397595STQ
CSB-BP397595STQ
CSB-MP397595STQ
CSB-EP397595STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Dihydrodipicolinate reductase (dapB) CSB-YP397596STQ
CSB-EP397596STQ
CSB-BP397596STQ
CSB-MP397596STQ
CSB-EP397596STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Chaperone protein DnaK (dnaK), partial CSB-YP397597STQ
CSB-EP397597STQ
CSB-BP397597STQ
CSB-MP397597STQ
CSB-EP397597STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP397598STQ
CSB-EP397598STQ
CSB-BP397598STQ
CSB-MP397598STQ
CSB-EP397598STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Pantothenate synthetase (panC) CSB-YP397599STQ
CSB-EP397599STQ
CSB-BP397599STQ
CSB-MP397599STQ
CSB-EP397599STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal protein S6 modification protein 2 (rimK2) CSB-YP397600STQ
CSB-EP397600STQ
CSB-BP397600STQ
CSB-MP397600STQ
CSB-EP397600STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 33 kDa chaperonin (hslO) CSB-YP397601STQ
CSB-EP397601STQ
CSB-BP397601STQ
CSB-MP397601STQ
CSB-EP397601STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Tyrosine recombinase XerC (xerC) CSB-YP397602STQ
CSB-EP397602STQ
CSB-BP397602STQ
CSB-MP397602STQ
CSB-EP397602STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP397603STQ
CSB-EP397603STQ
CSB-BP397603STQ
CSB-MP397603STQ
CSB-EP397603STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Acetylglutamate kinase (argB) CSB-YP397604STQ
CSB-EP397604STQ
CSB-BP397604STQ
CSB-MP397604STQ
CSB-EP397604STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L36 (rpmJ) CSB-YP397605STQ
CSB-EP397605STQ
CSB-BP397605STQ
CSB-MP397605STQ
CSB-EP397605STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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