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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium sp. Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP397245BPF
CSB-EP397245BPF
CSB-BP397245BPF
CSB-MP397245BPF
CSB-EP397245BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. UPF0753 protein BRADO2285 (BRADO2285), partial CSB-YP397246BPF
CSB-EP397246BPF
CSB-BP397246BPF
CSB-MP397246BPF
CSB-EP397246BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP397247BPF
CSB-EP397247BPF
CSB-BP397247BPF
CSB-MP397247BPF
CSB-EP397247BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S4 (rpsD) CSB-YP397248BPF
CSB-EP397248BPF
CSB-BP397248BPF
CSB-MP397248BPF
CSB-EP397248BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP397249BPF
CSB-EP397249BPF
CSB-BP397249BPF
CSB-MP397249BPF
CSB-EP397249BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP397250BPF
CSB-EP397250BPF
CSB-BP397250BPF
CSB-MP397250BPF
CSB-EP397250BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L16 (rplP) CSB-YP397251BPF
CSB-EP397251BPF
CSB-BP397251BPF
CSB-MP397251BPF
CSB-EP397251BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S8 (rpsH) CSB-YP397252BPF
CSB-EP397252BPF
CSB-BP397252BPF
CSB-MP397252BPF
CSB-EP397252BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP397253BPF
CSB-EP397253BPF
CSB-BP397253BPF
CSB-MP397253BPF
CSB-EP397253BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP397254BPF
CSB-EP397254BPF
CSB-BP397254BPF
CSB-MP397254BPF
CSB-EP397254BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Serine--tRNA ligase (serS) CSB-YP397255BPF
CSB-EP397255BPF
CSB-BP397255BPF
CSB-MP397255BPF
CSB-EP397255BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP397256BPF
CSB-EP397256BPF
CSB-BP397256BPF
CSB-MP397256BPF
CSB-EP397256BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP397257BPF
CSB-EP397257BPF
CSB-BP397257BPF
CSB-MP397257BPF
CSB-EP397257BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 30S ribosomal protein S2 (rpsB) CSB-YP397258BPF
CSB-EP397258BPF
CSB-BP397258BPF
CSB-MP397258BPF
CSB-EP397258BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Transcriptional repressor NrdR (nrdR) CSB-YP397259BPF
CSB-EP397259BPF
CSB-BP397259BPF
CSB-MP397259BPF
CSB-EP397259BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP397260BPF
CSB-EP397260BPF
CSB-BP397260BPF
CSB-MP397260BPF
CSB-EP397260BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable potassium transport system protein kup 3 (kup3), partial CSB-YP397261BPF
CSB-EP397261BPF
CSB-BP397261BPF
CSB-MP397261BPF
CSB-EP397261BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 2-isopropylmalate synthase (leuA), partial CSB-YP397262BPF
CSB-EP397262BPF
CSB-BP397262BPF
CSB-MP397262BPF
CSB-EP397262BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP397263BPF
CSB-EP397263BPF
CSB-BP397263BPF
CSB-MP397263BPF
CSB-EP397263BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Potassium-transporting ATPase C chain (kdpC), partial CSB-YP397264BPF
CSB-EP397264BPF
CSB-BP397264BPF
CSB-MP397264BPF
CSB-EP397264BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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