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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Clavibacter michiganensis subsp. michiganensis L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP397105CWT
CSB-EP397105CWT
CSB-BP397105CWT
CSB-MP397105CWT
CSB-EP397105CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Tryptophan synthase beta chain (trpB) CSB-YP397106CWT
CSB-EP397106CWT
CSB-BP397106CWT
CSB-MP397106CWT
CSB-EP397106CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Chorismate synthase (aroC) CSB-YP397107CWT
CSB-EP397107CWT
CSB-BP397107CWT
CSB-MP397107CWT
CSB-EP397107CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Alanine--tRNA ligase (alaS), partial CSB-YP397108CWT
CSB-EP397108CWT
CSB-BP397108CWT
CSB-MP397108CWT
CSB-EP397108CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Probable transcriptional regulatory protein CMM_1817 (CMM_1817) CSB-YP397109CWT
CSB-EP397109CWT
CSB-BP397109CWT
CSB-MP397109CWT
CSB-EP397109CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Cell division protein SepF (sepF) CSB-YP397110CWT
CSB-EP397110CWT
CSB-BP397110CWT
CSB-MP397110CWT
CSB-EP397110CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP397111CWT
CSB-EP397111CWT
CSB-BP397111CWT
CSB-MP397111CWT
CSB-EP397111CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP397112CWT
CSB-EP397112CWT
CSB-BP397112CWT
CSB-MP397112CWT
CSB-EP397112CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Dihydrodipicolinate reductase (dapB) CSB-YP397113CWT
CSB-EP397113CWT
CSB-BP397113CWT
CSB-MP397113CWT
CSB-EP397113CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Proline--tRNA ligase (proS), partial CSB-YP397114CWT
CSB-EP397114CWT
CSB-BP397114CWT
CSB-MP397114CWT
CSB-EP397114CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Na (+)/H (+) antiporter nhaA 2, partial CSB-YP397115CWT
CSB-EP397115CWT
CSB-BP397115CWT
CSB-MP397115CWT
CSB-EP397115CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Pantothenate kinase (coaA) CSB-YP397116CWT
CSB-EP397116CWT
CSB-BP397116CWT
CSB-MP397116CWT
CSB-EP397116CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Translation initiation factor IF-1 (infA) CSB-YP397117CWT
CSB-EP397117CWT
CSB-BP397117CWT
CSB-MP397117CWT
CSB-EP397117CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S3 (rpsC) CSB-YP397118CWT
CSB-EP397118CWT
CSB-BP397118CWT
CSB-MP397118CWT
CSB-EP397118CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UPF0234 protein CMM_2802 (CMM_2802) CSB-YP397119CWT
CSB-EP397119CWT
CSB-BP397119CWT
CSB-MP397119CWT
CSB-EP397119CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L28 (rpmB) CSB-YP397120CWT
CSB-EP397120CWT
CSB-BP397120CWT
CSB-MP397120CWT
CSB-EP397120CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 50S ribosomal protein L25 (rplY) CSB-YP397121VDY
CSB-EP397121VDY
CSB-BP397121VDY
CSB-MP397121VDY
CSB-EP397121VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Tryptophan synthase alpha chain (trpA) CSB-YP397122VDY
CSB-EP397122VDY
CSB-BP397122VDY
CSB-MP397122VDY
CSB-EP397122VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 50S ribosomal protein L28 (rpmB) CSB-YP397123VDY
CSB-EP397123VDY
CSB-BP397123VDY
CSB-MP397123VDY
CSB-EP397123VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP397124VDY
CSB-EP397124VDY
CSB-BP397124VDY
CSB-MP397124VDY
CSB-EP397124VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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