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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella putrefaciens Sulfurtransferase TusA homolog (tusA) CSB-YP397365STQ
CSB-EP397365STQ
CSB-BP397365STQ
CSB-MP397365STQ
CSB-EP397365STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein smg homolog (smg) CSB-YP397366STQ
CSB-EP397366STQ
CSB-BP397366STQ
CSB-MP397366STQ
CSB-EP397366STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP397367STQ
CSB-EP397367STQ
CSB-BP397367STQ
CSB-MP397367STQ
CSB-EP397367STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Fumarate reductase subunit D (frdD), partial CSB-YP397368STQ1
CSB-EP397368STQ1
CSB-BP397368STQ1
CSB-MP397368STQ1
CSB-EP397368STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens D-tyrosyl-tRNA (Tyr) deacylase CSB-YP397369STQ
CSB-EP397369STQ
CSB-BP397369STQ
CSB-MP397369STQ
CSB-EP397369STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP397370STQ
CSB-EP397370STQ
CSB-BP397370STQ
CSB-MP397370STQ
CSB-EP397370STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 2-isopropylmalate synthase (leuA), partial CSB-YP397371STQ
CSB-EP397371STQ
CSB-BP397371STQ
CSB-MP397371STQ
CSB-EP397371STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein translocase subunit SecA (secA), partial CSB-YP397372STQ
CSB-EP397372STQ
CSB-BP397372STQ
CSB-MP397372STQ
CSB-EP397372STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP397373STQ
CSB-EP397373STQ
CSB-BP397373STQ
CSB-MP397373STQ
CSB-EP397373STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 10 kDa chaperonin (groS) CSB-YP397374STQ
CSB-EP397374STQ
CSB-BP397374STQ
CSB-MP397374STQ
CSB-EP397374STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 30S ribosomal protein S6 (rpsF) CSB-YP397375STQ
CSB-EP397375STQ
CSB-BP397375STQ
CSB-MP397375STQ
CSB-EP397375STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Outer-membrane lipoprotein lolB (lolB) CSB-YP397376STQ
CSB-EP397376STQ
CSB-BP397376STQ
CSB-MP397376STQ
CSB-EP397376STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP397377STQ
CSB-EP397377STQ
CSB-BP397377STQ
CSB-MP397377STQ
CSB-EP397377STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L27 (rpmA) CSB-YP397378STQ
CSB-EP397378STQ
CSB-BP397378STQ
CSB-MP397378STQ
CSB-EP397378STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP397379STQ
CSB-EP397379STQ
CSB-BP397379STQ
CSB-MP397379STQ
CSB-EP397379STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Isoleucine--tRNA ligase (ileS), partial CSB-YP397380STQ
CSB-EP397380STQ
CSB-BP397380STQ
CSB-MP397380STQ
CSB-EP397380STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP397381STQ
CSB-EP397381STQ
CSB-BP397381STQ
CSB-MP397381STQ
CSB-EP397381STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP397382STQ
CSB-EP397382STQ
CSB-BP397382STQ
CSB-MP397382STQ
CSB-EP397382STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein grpE (grpE) CSB-YP397383STQ
CSB-EP397383STQ
CSB-BP397383STQ
CSB-MP397383STQ
CSB-EP397383STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protein Xni (xni) CSB-YP397384STQ
CSB-EP397384STQ
CSB-BP397384STQ
CSB-MP397384STQ
CSB-EP397384STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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