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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas mendocina Bifunctional protein HldE (hldE) CSB-YP397325PZM
CSB-EP397325PZM
CSB-BP397325PZM
CSB-MP397325PZM
CSB-EP397325PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Adenylosuccinate synthetase (purA) CSB-YP397326PZM
CSB-EP397326PZM
CSB-BP397326PZM
CSB-MP397326PZM
CSB-EP397326PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Urease accessory protein UreD (ureD) CSB-YP397327PZM
CSB-EP397327PZM
CSB-BP397327PZM
CSB-MP397327PZM
CSB-EP397327PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP397328PZM
CSB-EP397328PZM
CSB-BP397328PZM
CSB-MP397328PZM
CSB-EP397328PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP397329PZM
CSB-EP397329PZM
CSB-BP397329PZM
CSB-MP397329PZM
CSB-EP397329PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Isoleucine--tRNA ligase (ileS), partial CSB-YP397330PZM
CSB-EP397330PZM
CSB-BP397330PZM
CSB-MP397330PZM
CSB-EP397330PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L25 (rplY) CSB-YP397331PZM
CSB-EP397331PZM
CSB-BP397331PZM
CSB-MP397331PZM
CSB-EP397331PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Protein slyX homolog (slyX) CSB-YP397332PZM
CSB-EP397332PZM
CSB-BP397332PZM
CSB-MP397332PZM
CSB-EP397332PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Dihydroorotase (pyrC) CSB-YP397333PZM
CSB-EP397333PZM
CSB-BP397333PZM
CSB-MP397333PZM
CSB-EP397333PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP397334PZM
CSB-EP397334PZM
CSB-BP397334PZM
CSB-MP397334PZM
CSB-EP397334PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cobyric acid synthase (cobQ) CSB-YP397335PZM
CSB-EP397335PZM
CSB-BP397335PZM
CSB-MP397335PZM
CSB-EP397335PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Integration host factor subunit beta (ihfB) CSB-YP397336PZM
CSB-EP397336PZM
CSB-BP397336PZM
CSB-MP397336PZM
CSB-EP397336PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP397337PZM
CSB-EP397337PZM
CSB-BP397337PZM
CSB-MP397337PZM
CSB-EP397337PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L35 (rpmI) CSB-YP397338PZM
CSB-EP397338PZM
CSB-BP397338PZM
CSB-MP397338PZM
CSB-EP397338PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Putative phosphotransferase Pmen_2084 (Pmen_2084) CSB-YP397339PZM
CSB-EP397339PZM
CSB-BP397339PZM
CSB-MP397339PZM
CSB-EP397339PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UvrABC system protein C (uvrC), partial CSB-YP397340PZM
CSB-EP397340PZM
CSB-BP397340PZM
CSB-MP397340PZM
CSB-EP397340PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP397341PZM
CSB-EP397341PZM
CSB-BP397341PZM
CSB-MP397341PZM
CSB-EP397341PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina tRNA 2-selenouridine synthase (selU) CSB-YP397342PZM
CSB-EP397342PZM
CSB-BP397342PZM
CSB-MP397342PZM
CSB-EP397342PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP397343PZM
CSB-EP397343PZM
CSB-BP397343PZM
CSB-MP397343PZM
CSB-EP397343PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Serine/threonine transporter sstT (sstT), partial CSB-YP397344PZM
CSB-EP397344PZM
CSB-BP397344PZM
CSB-MP397344PZM
CSB-EP397344PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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