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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella putrefaciens 50S ribosomal protein L21 (rplU) CSB-YP397566STQ
CSB-EP397566STQ
CSB-BP397566STQ
CSB-MP397566STQ
CSB-EP397566STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP397567STQ
CSB-EP397567STQ
CSB-BP397567STQ
CSB-MP397567STQ
CSB-EP397567STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP397568STQ
CSB-EP397568STQ
CSB-BP397568STQ
CSB-MP397568STQ
CSB-EP397568STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens RNA pyrophosphohydrolase (rppH) CSB-YP397569STQ
CSB-EP397569STQ
CSB-BP397569STQ
CSB-MP397569STQ
CSB-EP397569STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP397570STQ
CSB-EP397570STQ
CSB-BP397570STQ
CSB-MP397570STQ
CSB-EP397570STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens tRNA sulfurtransferase (thiI) CSB-YP397571STQ
CSB-EP397571STQ
CSB-BP397571STQ
CSB-MP397571STQ
CSB-EP397571STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Homoserine O-succinyltransferase (metA) CSB-YP397572STQ
CSB-EP397572STQ
CSB-BP397572STQ
CSB-MP397572STQ
CSB-EP397572STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Bifunctional protein FolD (folD) CSB-YP397573STQ
CSB-EP397573STQ
CSB-BP397573STQ
CSB-MP397573STQ
CSB-EP397573STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0434 protein Sputcn32_1559 (Sputcn32_1559) CSB-YP397574STQ
CSB-EP397574STQ
CSB-BP397574STQ
CSB-MP397574STQ
CSB-EP397574STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP397575STQ
CSB-EP397575STQ
CSB-BP397575STQ
CSB-MP397575STQ
CSB-EP397575STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Fatty acid metabolism regulator protein (fadR) CSB-YP397576STQ
CSB-EP397576STQ
CSB-BP397576STQ
CSB-MP397576STQ
CSB-EP397576STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Asparagine--tRNA ligase (asnS) CSB-YP397577STQ
CSB-EP397577STQ
CSB-BP397577STQ
CSB-MP397577STQ
CSB-EP397577STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 7-cyano-7-deazaguanine synthase (queC) CSB-YP397578STQ
CSB-EP397578STQ
CSB-BP397578STQ
CSB-MP397578STQ
CSB-EP397578STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP397579STQ
CSB-EP397579STQ
CSB-BP397579STQ
CSB-MP397579STQ
CSB-EP397579STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Integration host factor subunit beta (ihfB) CSB-YP397580STQ
CSB-EP397580STQ
CSB-BP397580STQ
CSB-MP397580STQ
CSB-EP397580STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP397581STQ1
CSB-EP397581STQ1
CSB-BP397581STQ1
CSB-MP397581STQ1
CSB-EP397581STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0176 protein Sputcn32_2130 (Sputcn32_2130) CSB-YP397582STQ
CSB-EP397582STQ
CSB-BP397582STQ
CSB-MP397582STQ
CSB-EP397582STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Methionine--tRNA ligase (metG), partial CSB-YP397583STQ
CSB-EP397583STQ
CSB-BP397583STQ
CSB-MP397583STQ
CSB-EP397583STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens High frequency lysogenization protein HflD homolog (hflD) CSB-YP397584STQ
CSB-EP397584STQ
CSB-BP397584STQ
CSB-MP397584STQ
CSB-EP397584STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Protease HtpX (htpX), partial CSB-YP397585STQ1
CSB-EP397585STQ1
CSB-BP397585STQ1
CSB-MP397585STQ1
CSB-EP397585STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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