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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium sp. ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP398966BPE
CSB-EP398966BPE
CSB-BP398966BPE
CSB-MP398966BPE
CSB-EP398966BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP398967BPE
CSB-EP398967BPE
CSB-BP398967BPE
CSB-MP398967BPE
CSB-EP398967BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribonuclease PH (rph) CSB-YP398968BPE
CSB-EP398968BPE
CSB-BP398968BPE
CSB-MP398968BPE
CSB-EP398968BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L20 (rplT) CSB-YP398969BPE
CSB-EP398969BPE
CSB-BP398969BPE
CSB-MP398969BPE
CSB-EP398969BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP398970BPE
CSB-EP398970BPE
CSB-BP398970BPE
CSB-MP398970BPE
CSB-EP398970BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP398971BPE
CSB-EP398971BPE
CSB-BP398971BPE
CSB-MP398971BPE
CSB-EP398971BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable intracellular septation protein A (BBta_0376), partial CSB-YP398972BPE1
CSB-EP398972BPE1
CSB-BP398972BPE1
CSB-MP398972BPE1
CSB-EP398972BPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP synthase gamma chain (atpG) CSB-YP398973BPE
CSB-EP398973BPE
CSB-BP398973BPE
CSB-MP398973BPE
CSB-EP398973BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Glutamate 5-kinase (proB) CSB-YP398974BPE
CSB-EP398974BPE
CSB-BP398974BPE
CSB-MP398974BPE
CSB-EP398974BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Ribonuclease H (rnhA) CSB-YP398976BPE
CSB-EP398976BPE
CSB-BP398976BPE
CSB-MP398976BPE
CSB-EP398976BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP synthase subunit a 2 (atpB2), partial CSB-YP398977BPE1
CSB-EP398977BPE1
CSB-BP398977BPE1
CSB-MP398977BPE1
CSB-EP398977BPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. DNA mismatch repair protein mutL (mutL), partial CSB-YP398978BPE
CSB-EP398978BPE
CSB-BP398978BPE
CSB-MP398978BPE
CSB-EP398978BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Adenylosuccinate synthetase (purA) CSB-YP398979BPE
CSB-EP398979BPE
CSB-BP398979BPE
CSB-MP398979BPE
CSB-EP398979BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Chorismate synthase (aroC) CSB-YP398980BPE
CSB-EP398980BPE
CSB-BP398980BPE
CSB-MP398980BPE
CSB-EP398980BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP398981BPE
CSB-EP398981BPE
CSB-BP398981BPE
CSB-MP398981BPE
CSB-EP398981BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Adenine deaminase (ade), partial CSB-YP398982BPE
CSB-EP398982BPE
CSB-BP398982BPE
CSB-MP398982BPE
CSB-EP398982BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable potassium transport system protein kup 2 (kup2), partial CSB-YP398983BPE
CSB-EP398983BPE
CSB-BP398983BPE
CSB-MP398983BPE
CSB-EP398983BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. 50S ribosomal protein L9 (rplI) CSB-YP398984BPE
CSB-EP398984BPE
CSB-BP398984BPE
CSB-MP398984BPE
CSB-EP398984BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Probable cytosol aminopeptidase (pepA) CSB-YP398985BPE
CSB-EP398985BPE
CSB-BP398985BPE
CSB-MP398985BPE
CSB-EP398985BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Bifunctional enzyme IspD/IspF (ispDF) CSB-YP398986BPE
CSB-EP398986BPE
CSB-BP398986BPE
CSB-MP398986BPE
CSB-EP398986BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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