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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clavibacter michiganensis subsp. michiganensis Glutamate 5-kinase (proB) CSB-YP397305CWT
CSB-EP397305CWT
CSB-BP397305CWT
CSB-MP397305CWT
CSB-EP397305CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Leucine--tRNA ligase (leuS), partial CSB-YP397306CWT
CSB-EP397306CWT
CSB-BP397306CWT
CSB-MP397306CWT
CSB-EP397306CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 2-isopropylmalate synthase (leuA), partial CSB-YP397307CWT
CSB-EP397307CWT
CSB-BP397307CWT
CSB-MP397307CWT
CSB-EP397307CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UvrABC system protein B (uvrB), partial CSB-YP397308CWT
CSB-EP397308CWT
CSB-BP397308CWT
CSB-MP397308CWT
CSB-EP397308CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP397309CWT
CSB-EP397309CWT
CSB-BP397309CWT
CSB-MP397309CWT
CSB-EP397309CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Aspartate carbamoyltransferase (pyrB) CSB-YP397310CWT
CSB-EP397310CWT
CSB-BP397310CWT
CSB-MP397310CWT
CSB-EP397310CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP397311CWT
CSB-EP397311CWT
CSB-BP397311CWT
CSB-MP397311CWT
CSB-EP397311CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Anthranilate phosphoribosyltransferase (trpD) CSB-YP397312CWT
CSB-EP397312CWT
CSB-BP397312CWT
CSB-MP397312CWT
CSB-EP397312CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L35 (rpmI) CSB-YP397313CWT
CSB-EP397313CWT
CSB-BP397313CWT
CSB-MP397313CWT
CSB-EP397313CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP397314CWT
CSB-EP397314CWT
CSB-BP397314CWT
CSB-MP397314CWT
CSB-EP397314CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP397315CWT
CSB-EP397315CWT
CSB-BP397315CWT
CSB-MP397315CWT
CSB-EP397315CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Phosphoglucosamine mutase (glmM) CSB-YP397316CWT
CSB-EP397316CWT
CSB-BP397316CWT
CSB-MP397316CWT
CSB-EP397316CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Adenylate kinase (adk) CSB-YP397317CWT
CSB-EP397317CWT
CSB-BP397317CWT
CSB-MP397317CWT
CSB-EP397317CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S19 (rpsS) CSB-YP397318CWT
CSB-EP397318CWT
CSB-BP397318CWT
CSB-MP397318CWT
CSB-EP397318CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S12 (rpsL) CSB-YP397319CWT
CSB-EP397319CWT
CSB-BP397319CWT
CSB-MP397319CWT
CSB-EP397319CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S18 (rpsR) CSB-YP397320CWT
CSB-EP397320CWT
CSB-BP397320CWT
CSB-MP397320CWT
CSB-EP397320CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Agmatine deiminase (aguA) CSB-YP397321PZM
CSB-EP397321PZM
CSB-BP397321PZM
CSB-MP397321PZM
CSB-EP397321PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP397322PZM
CSB-EP397322PZM
CSB-BP397322PZM
CSB-MP397322PZM
CSB-EP397322PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina HTH-type transcriptional regulator BetI (betI) CSB-YP397323PZM
CSB-EP397323PZM
CSB-BP397323PZM
CSB-MP397323PZM
CSB-EP397323PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP397324PZM
CSB-EP397324PZM
CSB-BP397324PZM
CSB-MP397324PZM
CSB-EP397324PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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