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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Orientia tsutsugamushi Ferredoxin--NADP reductase (OTBS_1841) CSB-YP398126ODJ
CSB-EP398126ODJ
CSB-BP398126ODJ
CSB-MP398126ODJ
CSB-EP398126ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L27 (rpmA) CSB-YP398127ODJ
CSB-EP398127ODJ
CSB-BP398127ODJ
CSB-MP398127ODJ
CSB-EP398127ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Protein-export protein secB (secB) CSB-YP398128ODJ
CSB-EP398128ODJ
CSB-BP398128ODJ
CSB-MP398128ODJ
CSB-EP398128ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP398129CWT
CSB-EP398129CWT
CSB-BP398129CWT
CSB-MP398129CWT
CSB-EP398129CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UPF0060 membrane protein CMM_0279 (CMM_0279), partial CSB-YP398130CWT1
CSB-EP398130CWT1
CSB-BP398130CWT1
CSB-MP398130CWT1
CSB-EP398130CWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Glutamyl-tRNA reductase (hemA) CSB-YP398131CWT
CSB-EP398131CWT
CSB-BP398131CWT
CSB-MP398131CWT
CSB-EP398131CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Protein translocase subunit SecA (secA), partial CSB-YP398132CWT
CSB-EP398132CWT
CSB-BP398132CWT
CSB-MP398132CWT
CSB-EP398132CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP398133CWT
CSB-EP398133CWT
CSB-BP398133CWT
CSB-MP398133CWT
CSB-EP398133CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Gamma-glutamyl phosphate reductase (proA) CSB-YP398134CWT
CSB-EP398134CWT
CSB-BP398134CWT
CSB-MP398134CWT
CSB-EP398134CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Probable rRNA maturation factor (CMM_1569) CSB-YP398135CWT
CSB-EP398135CWT
CSB-BP398135CWT
CSB-MP398135CWT
CSB-EP398135CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Putative sporulation transcription regulator WhiA (whiA) CSB-YP398136CWT
CSB-EP398136CWT
CSB-BP398136CWT
CSB-MP398136CWT
CSB-EP398136CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP398137CWT
CSB-EP398137CWT
CSB-BP398137CWT
CSB-MP398137CWT
CSB-EP398137CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP398138CWT
CSB-EP398138CWT
CSB-BP398138CWT
CSB-MP398138CWT
CSB-EP398138CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Transcriptional repressor NrdR (nrdR) CSB-YP398139CWT
CSB-EP398139CWT
CSB-BP398139CWT
CSB-MP398139CWT
CSB-EP398139CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis LexA repressor (lexA) CSB-YP398140CWT
CSB-EP398140CWT
CSB-BP398140CWT
CSB-MP398140CWT
CSB-EP398140CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UPF0678 fatty acid-binding protein-like protein CMM_2499 (CMM_2499) CSB-YP398141CWT
CSB-EP398141CWT
CSB-BP398141CWT
CSB-MP398141CWT
CSB-EP398141CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Bifunctional protein FolD (folD) CSB-YP398142CWT
CSB-EP398142CWT
CSB-BP398142CWT
CSB-MP398142CWT
CSB-EP398142CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L36 1 (rpmJ1) CSB-YP398143CWT
CSB-EP398143CWT
CSB-BP398143CWT
CSB-MP398143CWT
CSB-EP398143CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L24 (rplX) CSB-YP398144CWT
CSB-EP398144CWT
CSB-BP398144CWT
CSB-MP398144CWT
CSB-EP398144CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP398145CWT
CSB-EP398145CWT
CSB-BP398145CWT
CSB-MP398145CWT
CSB-EP398145CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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